Intratunical injection of stromal vascular fraction prevents fibrosis in a rat model of Peyronie's disease

the Trauma and Reconstructive Urology Working Party of the European Association of Urology (EAU) Young Academic Urologists (YAU)

doi:10.1111/bju.14570

Intratunical injection of stromal vascular fraction prevents fibrosis in a rat model of Peyronie's disease

the Trauma and Reconstructive Urology Working Party of the European Association of Urology (EAU) Young Academic Urologists (YAU)

School of Medicine

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Objective: To investigate whether local injection of autologous adipose stromal vascular fraction (SVF) can prevent the development of fibrosis and elastosis in the tunica albuginea (TA) using a rat model of the acute phase of Peyronie's disease (PD). Methods: A total of 24 male 12-week-old Sprague–Dawley rats were divided into three equal groups: sham; PD without treatment (transforming growth factor-β [TGF -β]); and PD treated with SVF 1 day after disease induction. Sham rats received two injections of vehicle into the TA 1 day apart. TGF -β rats received TGF- β1 injection and injection of vehicle 1 day later. SVF rats received TGF-β1 injection, followed by SVF 1 day later. One month after treatment, all rats underwent measurement of intracavernosal pressure and mean arterial pressure during electrostimulation of the cavernous nerve. The rats were then killed and penises were harvested for histology and Western blot analysis. Results: Erectile function was moderately reduced in the TGF-β group and was significantly improved after SVF treatment (P < 0.05). PD rats developed areas of fibrosis with a significant upregulation of collagen III, collagen I and elastin protein expression. These fibrotic changes were prevented when treated with SVF. Conclusions: Local injection of SVF may represent treatment for the acute phase of PD.

Original language	English (US)
Pages (from-to)	342-348
Number of pages	7
Journal	BJU International
Volume	124
Issue number	2
DOIs	https://doi.org/10.1111/bju.14570
State	Published - Aug 2019

Keywords

adipose stem cell, #Peyronies, #erectiledysfunction
penile plaque
sexual dysfunction

ASJC Scopus subject areas

Urology

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10.1111/bju.14570

Cite this

@article{55e06511e3c3454989474e14de5bc6f9,

title = "Intratunical injection of stromal vascular fraction prevents fibrosis in a rat model of Peyronie's disease",

abstract = "Objective: To investigate whether local injection of autologous adipose stromal vascular fraction (SVF) can prevent the development of fibrosis and elastosis in the tunica albuginea (TA) using a rat model of the acute phase of Peyronie's disease (PD). Methods: A total of 24 male 12-week-old Sprague–Dawley rats were divided into three equal groups: sham; PD without treatment (transforming growth factor-β [TGF -β]); and PD treated with SVF 1 day after disease induction. Sham rats received two injections of vehicle into the TA 1 day apart. TGF -β rats received TGF- β1 injection and injection of vehicle 1 day later. SVF rats received TGF-β1 injection, followed by SVF 1 day later. One month after treatment, all rats underwent measurement of intracavernosal pressure and mean arterial pressure during electrostimulation of the cavernous nerve. The rats were then killed and penises were harvested for histology and Western blot analysis. Results: Erectile function was moderately reduced in the TGF-β group and was significantly improved after SVF treatment (P < 0.05). PD rats developed areas of fibrosis with a significant upregulation of collagen III, collagen I and elastin protein expression. These fibrotic changes were prevented when treated with SVF. Conclusions: Local injection of SVF may represent treatment for the acute phase of PD.",

keywords = "adipose stem cell, #Peyronies, #erectiledysfunction, penile plaque, sexual dysfunction",

author = "{the Trauma and Reconstructive Urology Working Party of the European Association of Urology (EAU) Young Academic Urologists (YAU)} and Fabio Castiglione and Petter Hedlund and Emmanuel Weyne and Lukman Hakim and Francesco Montorsi and Andrea Salonia and Bivalacqua, {Trinity J.} and {De Ridder}, Dirk and Uros Milenkovic and David Ralph and Giulio Garaffa and Asif Muneer and Steven Joniau and Maarten Albersen",

note = "Funding Information: This study was funded by the European Society for Sexual Medicine grant for basic medical research 2011 awarded to Fabio Castiglione and Maarten Albersen and by the European Urological Scholarship Programme awarded to Fabio Castiglione. Asif Muneer is supported by the National Institute for Health Research, Biomedical Research Centre at University College London Hospital. All authors declare no competing financial interests. Publisher Copyright: {\textcopyright} 2018 The Authors BJU International {\textcopyright} 2018 BJU International Published by John Wiley & Sons Ltd",

year = "2019",

month = aug,

doi = "10.1111/bju.14570",

language = "English (US)",

volume = "124",

pages = "342--348",

journal = "BJU International",

issn = "1464-4096",

publisher = "Wiley-Blackwell",

number = "2",

}

TY - JOUR

T1 - Intratunical injection of stromal vascular fraction prevents fibrosis in a rat model of Peyronie's disease

AU - the Trauma and Reconstructive Urology Working Party of the European Association of Urology (EAU) Young Academic Urologists (YAU)

AU - Castiglione, Fabio

AU - Hedlund, Petter

AU - Weyne, Emmanuel

AU - Hakim, Lukman

AU - Montorsi, Francesco

AU - Salonia, Andrea

AU - Bivalacqua, Trinity J.

AU - De Ridder, Dirk

AU - Milenkovic, Uros

AU - Ralph, David

AU - Garaffa, Giulio

AU - Muneer, Asif

AU - Joniau, Steven

AU - Albersen, Maarten

N1 - Funding Information: This study was funded by the European Society for Sexual Medicine grant for basic medical research 2011 awarded to Fabio Castiglione and Maarten Albersen and by the European Urological Scholarship Programme awarded to Fabio Castiglione. Asif Muneer is supported by the National Institute for Health Research, Biomedical Research Centre at University College London Hospital. All authors declare no competing financial interests. Publisher Copyright: © 2018 The Authors BJU International © 2018 BJU International Published by John Wiley & Sons Ltd

PY - 2019/8

Y1 - 2019/8

N2 - Objective: To investigate whether local injection of autologous adipose stromal vascular fraction (SVF) can prevent the development of fibrosis and elastosis in the tunica albuginea (TA) using a rat model of the acute phase of Peyronie's disease (PD). Methods: A total of 24 male 12-week-old Sprague–Dawley rats were divided into three equal groups: sham; PD without treatment (transforming growth factor-β [TGF -β]); and PD treated with SVF 1 day after disease induction. Sham rats received two injections of vehicle into the TA 1 day apart. TGF -β rats received TGF- β1 injection and injection of vehicle 1 day later. SVF rats received TGF-β1 injection, followed by SVF 1 day later. One month after treatment, all rats underwent measurement of intracavernosal pressure and mean arterial pressure during electrostimulation of the cavernous nerve. The rats were then killed and penises were harvested for histology and Western blot analysis. Results: Erectile function was moderately reduced in the TGF-β group and was significantly improved after SVF treatment (P < 0.05). PD rats developed areas of fibrosis with a significant upregulation of collagen III, collagen I and elastin protein expression. These fibrotic changes were prevented when treated with SVF. Conclusions: Local injection of SVF may represent treatment for the acute phase of PD.

AB - Objective: To investigate whether local injection of autologous adipose stromal vascular fraction (SVF) can prevent the development of fibrosis and elastosis in the tunica albuginea (TA) using a rat model of the acute phase of Peyronie's disease (PD). Methods: A total of 24 male 12-week-old Sprague–Dawley rats were divided into three equal groups: sham; PD without treatment (transforming growth factor-β [TGF -β]); and PD treated with SVF 1 day after disease induction. Sham rats received two injections of vehicle into the TA 1 day apart. TGF -β rats received TGF- β1 injection and injection of vehicle 1 day later. SVF rats received TGF-β1 injection, followed by SVF 1 day later. One month after treatment, all rats underwent measurement of intracavernosal pressure and mean arterial pressure during electrostimulation of the cavernous nerve. The rats were then killed and penises were harvested for histology and Western blot analysis. Results: Erectile function was moderately reduced in the TGF-β group and was significantly improved after SVF treatment (P < 0.05). PD rats developed areas of fibrosis with a significant upregulation of collagen III, collagen I and elastin protein expression. These fibrotic changes were prevented when treated with SVF. Conclusions: Local injection of SVF may represent treatment for the acute phase of PD.

KW - adipose stem cell, #Peyronies, #erectiledysfunction

KW - penile plaque

KW - sexual dysfunction

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U2 - 10.1111/bju.14570

DO - 10.1111/bju.14570

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AN - SCOPUS:85062937286

SN - 1464-4096

VL - 124

SP - 342

EP - 348

JO - BJU International

JF - BJU International

IS - 2

ER -

Intratunical injection of stromal vascular fraction prevents fibrosis in a rat model of Peyronie's disease

Abstract

Keywords

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