Intranodal immunization with tumor lysate-pulsed dendritic cells enhances protective antitumor immunity

Laura A. Lambert, Glen R. Gibson, Marybeth Maloney, Brigit Durell, Randolph J. Noelle, Richard J. Barth

Research output: Contribution to journalArticle

Abstract

We developed a technique for direct inguinal lymph node injection in mice to compare various routes of immunization with tumor lysate-pulsed dendritic cell (DC) vaccines. Syngeneic, bone marrow-derived, tumor lysate-pulsed DCs administered intranodally generated more potent protective antitumor immunity than s.c. or i.v. DC immunizations. Intranodal immunization with ovalbumin peptide-pulsed DCs induced significantly greater antigen-specific T-lymphocyte expansion in the spleen than either s.c. or i.v. immunization. Furthermore, a significantly more potent, antigen-specific TH1-type response to the ovalbumin peptide was induced by intranodal, compared with s.c. or i.v., immunization. Intranodal immunization, designed to enhance DC-T cell interaction in a lymphoid environment, optimizes induction of T lymphocyte-mediated protective antitumor immunity. These results support the use of intranodal immunization as a feasible and effective route of DC vaccine administration.

Original languageEnglish (US)
Pages (from-to)641-646
Number of pages6
JournalCancer Research
Volume61
Issue number2
StatePublished - Jan 15 2001

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Intranodal immunization with tumor lysate-pulsed dendritic cells enhances protective antitumor immunity'. Together they form a unique fingerprint.

  • Cite this

    Lambert, L. A., Gibson, G. R., Maloney, M., Durell, B., Noelle, R. J., & Barth, R. J. (2001). Intranodal immunization with tumor lysate-pulsed dendritic cells enhances protective antitumor immunity. Cancer Research, 61(2), 641-646.