Intranodal immunization with tumor lysate-pulsed dendritic cells enhances protective antitumor immunity

Laura A. Lambert, Glen R. Gibson, Marybeth Maloney, Brigit Durell, Randolph J. Noelle, Richard J. Barth

Research output: Contribution to journalArticlepeer-review

85 Scopus citations


We developed a technique for direct inguinal lymph node injection in mice to compare various routes of immunization with tumor lysate-pulsed dendritic cell (DC) vaccines. Syngeneic, bone marrow-derived, tumor lysate-pulsed DCs administered intranodally generated more potent protective antitumor immunity than s.c. or i.v. DC immunizations. Intranodal immunization with ovalbumin peptide-pulsed DCs induced significantly greater antigen-specific T-lymphocyte expansion in the spleen than either s.c. or i.v. immunization. Furthermore, a significantly more potent, antigen-specific TH1-type response to the ovalbumin peptide was induced by intranodal, compared with s.c. or i.v., immunization. Intranodal immunization, designed to enhance DC-T cell interaction in a lymphoid environment, optimizes induction of T lymphocyte-mediated protective antitumor immunity. These results support the use of intranodal immunization as a feasible and effective route of DC vaccine administration.

Original languageEnglish (US)
Pages (from-to)641-646
Number of pages6
JournalCancer Research
Issue number2
StatePublished - Jan 15 2001

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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