Abstract
Aluminum can facilitate Fe-mediated oxidative injury, which may contribute to Al neurotoxicity. It has been reported that Al potentiates Fe-induced oxidative stress in cultured granule cells, suggesting a mechanism for Al facilitation of Fe-mediated oxidative injury. However, the relationship of intracellular Al concentration to Fe-induced oxidative stress has not been reported. In the present study, neuronal oxidative stress and survival were investigated. Embryo rat hippocampal neuron cultures were treated with Al2(SO4)3 and/or FESO4. An ionophore, A23187, was utilized to facilitate cellular Al uptake. Intraneuronal Al concentration was ascertained by laser microprobe mass spectrometry (LMMS). Neuronal oxidative stress was measured by confocal laser scanning microscopy, using 2,7-dichlorofluorescin diacetate (DCFH-DA) as a probe. The study showed that neuronal Al uptake was facilitated by the ionophore and that an increase of intraneuronal Al concentration potentiated Fe-induced oxidative stress and neuronal death. The results indicate that Al potentiation of Fe-induced oxidative stress might contribute to Al facilitation of oxidative injury, and thus to Al neurotoxicity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 271-277 |
| Number of pages | 7 |
| Journal | Brain Research |
| Volume | 743 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - 1996 |
| Externally published | Yes |
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Keywords
- 2,7-dichlorofluorescin diacetate
- aluminum
- confocal laser scanning microscopy
- hippocampal neuron
- iron
- laser microprobe mass spectrometry
- oxidative stress
- reactive oxygen species
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Intraneuronal aluminum potentiates iron-induced oxidative stress in cultured rat hippocampal neurons. / Xie, Charles X.; Mattson, Mark P.; Lovell, Mark A.; Yokel, Robert A.
In: Brain Research, Vol. 743, No. 1-2, 1996, p. 271-277.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intraneuronal aluminum potentiates iron-induced oxidative stress in cultured rat hippocampal neurons
AU - Xie, Charles X.
AU - Mattson, Mark P.
AU - Lovell, Mark A.
AU - Yokel, Robert A.
PY - 1996
Y1 - 1996
N2 - Aluminum can facilitate Fe-mediated oxidative injury, which may contribute to Al neurotoxicity. It has been reported that Al potentiates Fe-induced oxidative stress in cultured granule cells, suggesting a mechanism for Al facilitation of Fe-mediated oxidative injury. However, the relationship of intracellular Al concentration to Fe-induced oxidative stress has not been reported. In the present study, neuronal oxidative stress and survival were investigated. Embryo rat hippocampal neuron cultures were treated with Al2(SO4)3 and/or FESO4. An ionophore, A23187, was utilized to facilitate cellular Al uptake. Intraneuronal Al concentration was ascertained by laser microprobe mass spectrometry (LMMS). Neuronal oxidative stress was measured by confocal laser scanning microscopy, using 2,7-dichlorofluorescin diacetate (DCFH-DA) as a probe. The study showed that neuronal Al uptake was facilitated by the ionophore and that an increase of intraneuronal Al concentration potentiated Fe-induced oxidative stress and neuronal death. The results indicate that Al potentiation of Fe-induced oxidative stress might contribute to Al facilitation of oxidative injury, and thus to Al neurotoxicity.
AB - Aluminum can facilitate Fe-mediated oxidative injury, which may contribute to Al neurotoxicity. It has been reported that Al potentiates Fe-induced oxidative stress in cultured granule cells, suggesting a mechanism for Al facilitation of Fe-mediated oxidative injury. However, the relationship of intracellular Al concentration to Fe-induced oxidative stress has not been reported. In the present study, neuronal oxidative stress and survival were investigated. Embryo rat hippocampal neuron cultures were treated with Al2(SO4)3 and/or FESO4. An ionophore, A23187, was utilized to facilitate cellular Al uptake. Intraneuronal Al concentration was ascertained by laser microprobe mass spectrometry (LMMS). Neuronal oxidative stress was measured by confocal laser scanning microscopy, using 2,7-dichlorofluorescin diacetate (DCFH-DA) as a probe. The study showed that neuronal Al uptake was facilitated by the ionophore and that an increase of intraneuronal Al concentration potentiated Fe-induced oxidative stress and neuronal death. The results indicate that Al potentiation of Fe-induced oxidative stress might contribute to Al facilitation of oxidative injury, and thus to Al neurotoxicity.
KW - 2,7-dichlorofluorescin diacetate
KW - aluminum
KW - confocal laser scanning microscopy
KW - hippocampal neuron
KW - iron
KW - laser microprobe mass spectrometry
KW - oxidative stress
KW - reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=0030471998&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030471998&partnerID=8YFLogxK
U2 - 10.1016/S0006-8993(96)01055-4
DO - 10.1016/S0006-8993(96)01055-4
M3 - Article
C2 - 9017255
AN - SCOPUS:0030471998
VL - 743
SP - 271
EP - 277
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -