Intraneuronal aluminum potentiates iron-induced oxidative stress in cultured rat hippocampal neurons

Charles X. Xie, Mark P. Mattson, Mark A. Lovell, Robert A. Yokel

Research output: Contribution to journalArticlepeer-review


Aluminum can facilitate Fe-mediated oxidative injury, which may contribute to Al neurotoxicity. It has been reported that Al potentiates Fe-induced oxidative stress in cultured granule cells, suggesting a mechanism for Al facilitation of Fe-mediated oxidative injury. However, the relationship of intracellular Al concentration to Fe-induced oxidative stress has not been reported. In the present study, neuronal oxidative stress and survival were investigated. Embryo rat hippocampal neuron cultures were treated with Al2(SO4)3 and/or FESO4. An ionophore, A23187, was utilized to facilitate cellular Al uptake. Intraneuronal Al concentration was ascertained by laser microprobe mass spectrometry (LMMS). Neuronal oxidative stress was measured by confocal laser scanning microscopy, using 2,7-dichlorofluorescin diacetate (DCFH-DA) as a probe. The study showed that neuronal Al uptake was facilitated by the ionophore and that an increase of intraneuronal Al concentration potentiated Fe-induced oxidative stress and neuronal death. The results indicate that Al potentiation of Fe-induced oxidative stress might contribute to Al facilitation of oxidative injury, and thus to Al neurotoxicity.

Original languageEnglish (US)
Pages (from-to)271-277
Number of pages7
JournalBrain Research
Issue number1-2
StatePublished - 1996
Externally publishedYes


  • 2,7-dichlorofluorescin diacetate
  • aluminum
  • confocal laser scanning microscopy
  • hippocampal neuron
  • iron
  • laser microprobe mass spectrometry
  • oxidative stress
  • reactive oxygen species

ASJC Scopus subject areas

  • Neuroscience(all)


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