Intramolecular crosslink of human hemoglobin with a long chain dicarboxylic acid between the α subunits

H. Kwansa; A. Razynska; J. H. Collins; E. Bucci

Intramolecular crosslink of human hemoglobin with a long chain dicarboxylic acid between the α subunits

H. Kwansa, A. Razynska, J. H. Collins, E. Bucci

School of Medicine

Research output: Contribution to journal › Conference article › peer-review

Abstract

Crosslinks between the β82 lysines using 10, 12, and 14 carbon dicarboxylic acids is reported. It was found that the incubation mixture of human deoxyhemoglobin with bis- (3,5-dibromosalicyl)sebacate contains three main intramolecularly crosslinked molecular species. The major component is a hemoglobin crosslinked between the β82 lysines. These species have low oxygen affinity. Preliminary data suggest that the α subunits are crosslinked between the α99 residues.

Original language	English (US)
Pages (from-to)	A122
Journal	Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume	22
Issue number	5
State	Published - Nov 1 1994
Event	Proceedings of the 11th Congress of the International Society for Artificial Cells, Blood Substitutes and Immobilization Biotechnology, (ISABI) - Boston, MA, USA Duration: Jul 24 1994 → Jul 27 1994

ASJC Scopus subject areas

Biotechnology
Biomedical Engineering

Cite this

@article{98c0d61a4fdc46ff8d78b57de9d1cf63,

title = "Intramolecular crosslink of human hemoglobin with a long chain dicarboxylic acid between the α subunits",

abstract = "Crosslinks between the β82 lysines using 10, 12, and 14 carbon dicarboxylic acids is reported. It was found that the incubation mixture of human deoxyhemoglobin with bis- (3,5-dibromosalicyl)sebacate contains three main intramolecularly crosslinked molecular species. The major component is a hemoglobin crosslinked between the β82 lysines. These species have low oxygen affinity. Preliminary data suggest that the α subunits are crosslinked between the α99 residues.",

author = "H. Kwansa and A. Razynska and Collins, {J. H.} and E. Bucci",

year = "1994",

month = nov,

day = "1",

language = "English (US)",

volume = "22",

pages = "A122",

journal = "Artificial Cells, Blood Substitutes, and Immobilization Biotechnology",

issn = "1073-1199",

publisher = "Informa Healthcare",

number = "5",

note = "Proceedings of the 11th Congress of the International Society for Artificial Cells, Blood Substitutes and Immobilization Biotechnology, (ISABI) ; Conference date: 24-07-1994 Through 27-07-1994",

}

TY - JOUR

T1 - Intramolecular crosslink of human hemoglobin with a long chain dicarboxylic acid between the α subunits

AU - Kwansa, H.

AU - Razynska, A.

AU - Collins, J. H.

AU - Bucci, E.

PY - 1994/11/1

Y1 - 1994/11/1

N2 - Crosslinks between the β82 lysines using 10, 12, and 14 carbon dicarboxylic acids is reported. It was found that the incubation mixture of human deoxyhemoglobin with bis- (3,5-dibromosalicyl)sebacate contains three main intramolecularly crosslinked molecular species. The major component is a hemoglobin crosslinked between the β82 lysines. These species have low oxygen affinity. Preliminary data suggest that the α subunits are crosslinked between the α99 residues.

AB - Crosslinks between the β82 lysines using 10, 12, and 14 carbon dicarboxylic acids is reported. It was found that the incubation mixture of human deoxyhemoglobin with bis- (3,5-dibromosalicyl)sebacate contains three main intramolecularly crosslinked molecular species. The major component is a hemoglobin crosslinked between the β82 lysines. These species have low oxygen affinity. Preliminary data suggest that the α subunits are crosslinked between the α99 residues.

UR - http://www.scopus.com/inward/record.url?scp=0028533845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028533845&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:0028533845

SN - 1073-1199

VL - 22

SP - A122

JO - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology

JF - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology

IS - 5

T2 - Proceedings of the 11th Congress of the International Society for Artificial Cells, Blood Substitutes and Immobilization Biotechnology, (ISABI)

Y2 - 24 July 1994 through 27 July 1994

ER -

Intramolecular crosslink of human hemoglobin with a long chain dicarboxylic acid between the α subunits

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this