Intrahippocampal injection of pertussis toxin blocks adenosine suppression of synaptic responses

Kathleen R. Stratton; Andrew J. Cole; John Pritchett; Christine U. Eccles; Paul F. Worley; Jay M. Baraban

doi:10.1016/0006-8993(89)90604-5

Intrahippocampal injection of pertussis toxin blocks adenosine suppression of synaptic responses

Kathleen R. Stratton, Andrew J. Cole, John Pritchett, Christine U. Eccles, Paul F. Worley, Jay M. Baraban

School of Medicine

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Adenosine exerts prominent inhibitory effects on synaptic transmission via a presynaptic action. Using the hippocampal slice preparation, we have found in electrophysiological experiments that this action of adenosine is blocked by intrahippocampal injections of pertussis toxin. In biochemical studies, we have confirmed that this treatment affects the GTP-binding proteins, G_i and G_o, in this preparation. These results indicate that both pre- and postsynaptic actions of adenosine involve pertussis toxin-sensitive GTP-binding proteins.

Original language	English (US)
Pages (from-to)	359-364
Number of pages	6
Journal	Brain research
Volume	494
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(89)90604-5
State	Published - Aug 14 1989

Keywords

Adenosine
Caffeine
Guanosine triphosphate-binding protein
Hippocampus
Pertussis toxin

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(89)90604-5

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title = "Intrahippocampal injection of pertussis toxin blocks adenosine suppression of synaptic responses",

abstract = "Adenosine exerts prominent inhibitory effects on synaptic transmission via a presynaptic action. Using the hippocampal slice preparation, we have found in electrophysiological experiments that this action of adenosine is blocked by intrahippocampal injections of pertussis toxin. In biochemical studies, we have confirmed that this treatment affects the GTP-binding proteins, Gi and Go, in this preparation. These results indicate that both pre- and postsynaptic actions of adenosine involve pertussis toxin-sensitive GTP-binding proteins.",

keywords = "Adenosine, Caffeine, Guanosine triphosphate-binding protein, Hippocampus, Pertussis toxin",

author = "Stratton, {Kathleen R.} and Cole, {Andrew J.} and John Pritchett and Eccles, {Christine U.} and Worley, {Paul F.} and Baraban, {Jay M.}",

note = "Funding Information: We thank D. Lawrence for excellent secretarial assistance. This work was supported by PHS Grants DA-00266 (J.M.B.), AG-00256 (P.EW.), MH-15330-10 (K.R.S.), and grants from the Epilepsy Foundation of America (A.J.C. and J.H.B.), Lucille P. Markey Charitable Trust (J.M.B,), the Sloan Foundation (J.M.B.), the Blades Center for Research on Alcoholism, and the Klingenstein Foundation (P.EW.).",

year = "1989",

month = aug,

day = "14",

doi = "10.1016/0006-8993(89)90604-5",

language = "English (US)",

volume = "494",

pages = "359--364",

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T1 - Intrahippocampal injection of pertussis toxin blocks adenosine suppression of synaptic responses

AU - Stratton, Kathleen R.

AU - Cole, Andrew J.

AU - Pritchett, John

AU - Eccles, Christine U.

AU - Worley, Paul F.

AU - Baraban, Jay M.

N1 - Funding Information: We thank D. Lawrence for excellent secretarial assistance. This work was supported by PHS Grants DA-00266 (J.M.B.), AG-00256 (P.EW.), MH-15330-10 (K.R.S.), and grants from the Epilepsy Foundation of America (A.J.C. and J.H.B.), Lucille P. Markey Charitable Trust (J.M.B,), the Sloan Foundation (J.M.B.), the Blades Center for Research on Alcoholism, and the Klingenstein Foundation (P.EW.).

PY - 1989/8/14

Y1 - 1989/8/14

N2 - Adenosine exerts prominent inhibitory effects on synaptic transmission via a presynaptic action. Using the hippocampal slice preparation, we have found in electrophysiological experiments that this action of adenosine is blocked by intrahippocampal injections of pertussis toxin. In biochemical studies, we have confirmed that this treatment affects the GTP-binding proteins, Gi and Go, in this preparation. These results indicate that both pre- and postsynaptic actions of adenosine involve pertussis toxin-sensitive GTP-binding proteins.

AB - Adenosine exerts prominent inhibitory effects on synaptic transmission via a presynaptic action. Using the hippocampal slice preparation, we have found in electrophysiological experiments that this action of adenosine is blocked by intrahippocampal injections of pertussis toxin. In biochemical studies, we have confirmed that this treatment affects the GTP-binding proteins, Gi and Go, in this preparation. These results indicate that both pre- and postsynaptic actions of adenosine involve pertussis toxin-sensitive GTP-binding proteins.

KW - Adenosine

KW - Caffeine

KW - Guanosine triphosphate-binding protein

KW - Hippocampus

KW - Pertussis toxin

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DO - 10.1016/0006-8993(89)90604-5

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EP - 364

JO - Brain research

JF - Brain research

IS - 2

ER -

Intrahippocampal injection of pertussis toxin blocks adenosine suppression of synaptic responses

Abstract

Keywords

ASJC Scopus subject areas

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