Intracellular volume and apparent diffusion constants of perfused cancer cell cultures, as measured by NMR

Ulrich Pilatus, Hyunsuk Shim, Dmitri Artemov, Delphine Davis, Peter C Van Zijl, Jerry D. Glickson

Research output: Contribution to journalArticle

Abstract

Diffusion NMR spectroscopy was used to study intracellular volume and apparent water diffusion constants in different cell lines (DU145, human prostate cancer; AT3, rat prostate cancer; MCF-7, human breast cancer; RIF- 1, mouse fibrosacroma). The cells were grown on various matrices (collagen sponge, collagen beads, polystyrene beads) which enabled continuous growth in perfused high density cell culture suitable for NMR studies. In perfused cell systems, the attenuation of the water signal versus the squared gradient strength was fitted by the sum of two decaying exponentials. For the slowly decaying component the apparent water diffusion constant at 37°C was 0.22 (±0.02) x 10-9 s/m2 for all cell lines at diffusion times > 100 ms. It continuously increased up to 0.47(±0.05) x 10-9 s/m2 when the diffusion time was decreased to 8 ms, indicating restricted diffusion. No significant effect of the matrices was observed. The fractional volume of the slew component as determined from the biexponential diffusion curve correlated with the relative intracellular volume, as obtained from the cell density in the sample and the cell size as measured by light microsocopy. Therefore, this simple NMR approach can be used to determine intracellular volume in perfused cell cultures suitable for NMR studies. Using this information in combination with spectroscopic data, changes in intracellular metabolite concentration can be detected even when the cellular volume is changing during the experiment. The apparent diffusion constant for the fast diffusing component varied with growth matrix, cell density and cell type and also showed the typical characteristics of restricted diffusion (increase of apparent diffusion constant with time).

Original languageEnglish (US)
Pages (from-to)825-832
Number of pages8
JournalMagnetic Resonance in Medicine
Volume37
Issue number6
DOIs
StatePublished - Jun 1997

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Cell Culture Techniques
Neoplasms
Water
Prostatic Neoplasms
Collagen
Cell Count
Cell Line
Polystyrenes
Porifera
Growth
Cell Size
Sample Size
Magnetic Resonance Spectroscopy
Breast Neoplasms
Light

Keywords

  • Apparent diffusion constant
  • Cell volume
  • Diffusion-weighted NMR spectroscopy
  • Microcarrier cell culture

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Intracellular volume and apparent diffusion constants of perfused cancer cell cultures, as measured by NMR. / Pilatus, Ulrich; Shim, Hyunsuk; Artemov, Dmitri; Davis, Delphine; Van Zijl, Peter C; Glickson, Jerry D.

In: Magnetic Resonance in Medicine, Vol. 37, No. 6, 06.1997, p. 825-832.

Research output: Contribution to journalArticle

Pilatus, Ulrich ; Shim, Hyunsuk ; Artemov, Dmitri ; Davis, Delphine ; Van Zijl, Peter C ; Glickson, Jerry D. / Intracellular volume and apparent diffusion constants of perfused cancer cell cultures, as measured by NMR. In: Magnetic Resonance in Medicine. 1997 ; Vol. 37, No. 6. pp. 825-832.
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