Intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site

Erik Lontok, Emily Corse, Carolyn E Machamer

Research output: Contribution to journalArticle

Abstract

Coronavirus budding at the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) requires accumulation of the viral envelope proteins at this point in the secretory pathway. Here we demonstrate that the spike (S) protein from the group 3 coronavirus infectious bronchitis virus (IBV) contains a canonical dilysine endoplasmic reticulum retrieval signal (-KKXX-COOH) in its cytoplasmic tail. This signal can retain a chimeric reporter protein in the ERGIC and when mutated allows transport of the full-length S protein as well as the chimera to the plasma membrane. Interestingly, the IBV S protein also contains a tyrosine-based endocytosis signal in its cytoplasmic tail, suggesting that any S protein that escapes the ERGIC will be rapidly endocytosed when it reaches the plasma membrane. We also identified a novel dibasic motif (-KXHXX-COOH) in the cytoplasmic tails of S proteins from group 1 coronaviruses and from the newly identified coronavirus implicated in severe acute respiratory syndrome. This dibasic motif also retained a reporter protein in the ERGIC, similar to the dilysine motif in IBV S. The cytoplasmic tails of S proteins from group 2 coronaviruses lack an intracellular localization signal. The inherent differences in S-protein trafficking could point to interesting variations in pathogenesis of coronaviruses, since increased levels of surface S protein could promote syncytium formation and direct cell-to-cell spread of the infection.

Original languageEnglish (US)
Pages (from-to)5913-5922
Number of pages10
JournalJournal of Virology
Volume78
Issue number11
DOIs
StatePublished - Jun 2004

Fingerprint

Coronavirus Spike Glycoproteins
Virus Assembly
Coronavirus
Coronavirinae
Protein S
Endoplasmic Reticulum
Infectious bronchitis virus
endoplasmic reticulum
lysyllysine
tail
proteins
Endocytosis
Cell Membrane
Viral Envelope Proteins
Severe Acute Respiratory Syndrome
plasma membrane
Secretory Pathway
Protein Transport
recombinant fusion proteins
Giant Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site. / Lontok, Erik; Corse, Emily; Machamer, Carolyn E.

In: Journal of Virology, Vol. 78, No. 11, 06.2004, p. 5913-5922.

Research output: Contribution to journalArticle

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