Intracellular pH determination by 13C-NMR spectroscopy

V. P. Chacko, Robert George Weiss

Research output: Contribution to journalArticle

Abstract

A noninvasive method for the determination of pH by the 13C-nuclear magnetic resonance (NMR) chemical shift of the C-3 carbon of sn-glycerol 3- phosphate is described. Nonlinear least-squares analysis of the chemical shift variation of the C-3 resonance of sn-glycerol 3-phosphate with pH at 37°C in solutions and in perchloric acid extracts of tissue yielded a pK(a) of 6.2, making it a very sensitive indicator of pH in the approximate range of 5-7. Intracellular pH determined by the present 13C-NMR method correlated well with simultaneous measurements of pH by 31P-NMR spectroscopy over a wide range during normal perfusion and ischemic conditions in intact rat hearts. These findings indicate that this approach is particularly suited for quantification of intracellular pH over the physiological range in intact tissues and that observed in ischemic myocardium.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume264
Issue number3 33-3
StatePublished - 1993

Fingerprint

Chemical shift
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Tissue Extracts
Rats
Carbon
Tissue
Least-Squares Analysis
alpha-glycerophosphoric acid
Myocardium
Reference Values
Perfusion
Perchloric Acid

Keywords

  • glycerol 3- phosphate
  • intermediary metabolism
  • ischemia
  • nuclear magnetic resonance

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Intracellular pH determination by 13C-NMR spectroscopy. / Chacko, V. P.; Weiss, Robert George.

In: American Journal of Physiology - Cell Physiology, Vol. 264, No. 3 33-3, 1993.

Research output: Contribution to journalArticle

@article{5acf6f1b3f314af0832f99cd83975bef,
title = "Intracellular pH determination by 13C-NMR spectroscopy",
abstract = "A noninvasive method for the determination of pH by the 13C-nuclear magnetic resonance (NMR) chemical shift of the C-3 carbon of sn-glycerol 3- phosphate is described. Nonlinear least-squares analysis of the chemical shift variation of the C-3 resonance of sn-glycerol 3-phosphate with pH at 37°C in solutions and in perchloric acid extracts of tissue yielded a pK(a) of 6.2, making it a very sensitive indicator of pH in the approximate range of 5-7. Intracellular pH determined by the present 13C-NMR method correlated well with simultaneous measurements of pH by 31P-NMR spectroscopy over a wide range during normal perfusion and ischemic conditions in intact rat hearts. These findings indicate that this approach is particularly suited for quantification of intracellular pH over the physiological range in intact tissues and that observed in ischemic myocardium.",
keywords = "glycerol 3- phosphate, intermediary metabolism, ischemia, nuclear magnetic resonance",
author = "Chacko, {V. P.} and Weiss, {Robert George}",
year = "1993",
language = "English (US)",
volume = "264",
journal = "American Journal of Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "3 33-3",

}

TY - JOUR

T1 - Intracellular pH determination by 13C-NMR spectroscopy

AU - Chacko, V. P.

AU - Weiss, Robert George

PY - 1993

Y1 - 1993

N2 - A noninvasive method for the determination of pH by the 13C-nuclear magnetic resonance (NMR) chemical shift of the C-3 carbon of sn-glycerol 3- phosphate is described. Nonlinear least-squares analysis of the chemical shift variation of the C-3 resonance of sn-glycerol 3-phosphate with pH at 37°C in solutions and in perchloric acid extracts of tissue yielded a pK(a) of 6.2, making it a very sensitive indicator of pH in the approximate range of 5-7. Intracellular pH determined by the present 13C-NMR method correlated well with simultaneous measurements of pH by 31P-NMR spectroscopy over a wide range during normal perfusion and ischemic conditions in intact rat hearts. These findings indicate that this approach is particularly suited for quantification of intracellular pH over the physiological range in intact tissues and that observed in ischemic myocardium.

AB - A noninvasive method for the determination of pH by the 13C-nuclear magnetic resonance (NMR) chemical shift of the C-3 carbon of sn-glycerol 3- phosphate is described. Nonlinear least-squares analysis of the chemical shift variation of the C-3 resonance of sn-glycerol 3-phosphate with pH at 37°C in solutions and in perchloric acid extracts of tissue yielded a pK(a) of 6.2, making it a very sensitive indicator of pH in the approximate range of 5-7. Intracellular pH determined by the present 13C-NMR method correlated well with simultaneous measurements of pH by 31P-NMR spectroscopy over a wide range during normal perfusion and ischemic conditions in intact rat hearts. These findings indicate that this approach is particularly suited for quantification of intracellular pH over the physiological range in intact tissues and that observed in ischemic myocardium.

KW - glycerol 3- phosphate

KW - intermediary metabolism

KW - ischemia

KW - nuclear magnetic resonance

UR - http://www.scopus.com/inward/record.url?scp=0027411297&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027411297&partnerID=8YFLogxK

M3 - Article

C2 - 8460678

AN - SCOPUS:0027411297

VL - 264

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6135

IS - 3 33-3

ER -