Intracellular oxygen: Similar results from two methods of measurement using phosphorescent nanoparticles

David Lloyd, Catrin F. Williams, K. Vijayalakshmi, M. Kombrabail, Nick White, Anthony J. Hayes, Miguel A. Aon, G. Krishnamoorthy

Research output: Contribution to journalArticle

Abstract

The ability to resolve the spatio-temporal complexity of intracellular O2 distribution is the "Holy Grail" of cellular physiology. In an effort to obtain a minimally invasive approach to the mapping of intracellular O2 tensions, two methods of phosphorescent lifetime imaging microscopy were compared in the current study and gave similar results. These were two-photon confocal laser scanning microscopy with pinhole shifting, and picosecond time-resolved epi-phosphorescence microscopy using a single 0.5 μm focused spot. Both methods utilized Ru coordination complex embedded nanoparticles (45 nm diameter) as the phosphorescent probe, excited using pulsed outputs of a titanium-sapphire Tsunami lasers (710-1050 nm).

Original languageEnglish (US)
Article number1350041
JournalJournal of Innovative Optical Health Sciences
Volume7
Issue number2
DOIs
StatePublished - Mar 2014

Keywords

  • Time-resolved phosphorescence
  • pinhole shifting
  • two-photon excitation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Medicine (miscellaneous)
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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    Lloyd, D., Williams, C. F., Vijayalakshmi, K., Kombrabail, M., White, N., Hayes, A. J., Aon, M. A., & Krishnamoorthy, G. (2014). Intracellular oxygen: Similar results from two methods of measurement using phosphorescent nanoparticles. Journal of Innovative Optical Health Sciences, 7(2), [1350041]. https://doi.org/10.1142/S1793545813500417