Intracellular oxygen: Similar results from two methods of measurement using phosphorescent nanoparticles

David Lloyd; Catrin F. Williams; K. Vijayalakshmi; M. Kombrabail; Nick White; Anthony J. Hayes; Miguel A. Aon; G. Krishnamoorthy

doi:10.1142/S1793545813500417

Intracellular oxygen: Similar results from two methods of measurement using phosphorescent nanoparticles

David Lloyd, Catrin F. Williams, K. Vijayalakshmi, M. Kombrabail, Nick White, Anthony J. Hayes, Miguel A. Aon, G. Krishnamoorthy

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The ability to resolve the spatio-temporal complexity of intracellular O₂ distribution is the "Holy Grail" of cellular physiology. In an effort to obtain a minimally invasive approach to the mapping of intracellular O₂ tensions, two methods of phosphorescent lifetime imaging microscopy were compared in the current study and gave similar results. These were two-photon confocal laser scanning microscopy with pinhole shifting, and picosecond time-resolved epi-phosphorescence microscopy using a single 0.5 μm focused spot. Both methods utilized Ru coordination complex embedded nanoparticles (45 nm diameter) as the phosphorescent probe, excited using pulsed outputs of a titanium-sapphire Tsunami lasers (710-1050 nm).

Original language	English (US)
Article number	1350041
Journal	Journal of Innovative Optical Health Sciences
Volume	7
Issue number	2
DOIs	https://doi.org/10.1142/S1793545813500417
State	Published - Mar 2014
Externally published	Yes

Keywords

Time-resolved phosphorescence
pinhole shifting
two-photon excitation

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Medicine (miscellaneous)
Atomic and Molecular Physics, and Optics
Biomedical Engineering

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10.1142/S1793545813500417

Cite this

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title = "Intracellular oxygen: Similar results from two methods of measurement using phosphorescent nanoparticles",

abstract = "The ability to resolve the spatio-temporal complexity of intracellular O2 distribution is the {"}Holy Grail{"} of cellular physiology. In an effort to obtain a minimally invasive approach to the mapping of intracellular O2 tensions, two methods of phosphorescent lifetime imaging microscopy were compared in the current study and gave similar results. These were two-photon confocal laser scanning microscopy with pinhole shifting, and picosecond time-resolved epi-phosphorescence microscopy using a single 0.5 μm focused spot. Both methods utilized Ru coordination complex embedded nanoparticles (45 nm diameter) as the phosphorescent probe, excited using pulsed outputs of a titanium-sapphire Tsunami lasers (710-1050 nm).",

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language = "English (US)",

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T2 - Similar results from two methods of measurement using phosphorescent nanoparticles

AU - Lloyd, David

AU - Williams, Catrin F.

AU - Vijayalakshmi, K.

AU - Kombrabail, M.

AU - White, Nick

AU - Hayes, Anthony J.

AU - Aon, Miguel A.

AU - Krishnamoorthy, G.

PY - 2014/3

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AB - The ability to resolve the spatio-temporal complexity of intracellular O2 distribution is the "Holy Grail" of cellular physiology. In an effort to obtain a minimally invasive approach to the mapping of intracellular O2 tensions, two methods of phosphorescent lifetime imaging microscopy were compared in the current study and gave similar results. These were two-photon confocal laser scanning microscopy with pinhole shifting, and picosecond time-resolved epi-phosphorescence microscopy using a single 0.5 μm focused spot. Both methods utilized Ru coordination complex embedded nanoparticles (45 nm diameter) as the phosphorescent probe, excited using pulsed outputs of a titanium-sapphire Tsunami lasers (710-1050 nm).

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KW - pinhole shifting

KW - two-photon excitation

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Intracellular oxygen: Similar results from two methods of measurement using phosphorescent nanoparticles

Abstract

Keywords

ASJC Scopus subject areas

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