Intracellular neutralization of a virus using a cell-penetrating molecular transporter

Gopal Sapparapu, Artez L. Sims, Mohammed S. Aiyegbo, Fyza Y. Shaikh, Eva M. Harth, James E. Crowe

Research output: Contribution to journalArticlepeer-review

Abstract

Aims: Antibodies are the principal mediator of immunity against reinfection with viruses. Antibodies typically neutralize viruses by binding to virion particles in solution prior to attachment to susceptible cells. Once viruses enter cells, conventional antibodies cannot inhibit virus infection or replication. It is desirable to develop an efficient and nontoxic method for the introduction of virus-inhibiting antibodies into cells.

Materials and methods: In this article, we report a new method for the delivery of small recombinant antibody fragments into virus-infected cells using a dendrimer-based molecular transporter.

Results and Conclusion: The construct penetrated virus-infected cells efficiently and inhibited virus replication. This method provides a novel approach for the immediate delivery of inhibitory antibodies directed to virus proteins that are exposed only in the intracellular environment. This approach circumvents the current and rather complicated expression of inhibitory antibodies in cells following gene transfer. Original submitted 9 March 2013; Revised submitted 8 July 201.

Original languageEnglish (US)
Pages (from-to)1613-1624
Number of pages12
JournalNanomedicine
Volume9
Issue number11
DOIs
StatePublished - Aug 1 2014
Externally publishedYes

Keywords

  • antiviral agents
  • dendrimers
  • intracellular neutralization
  • molecular transporter
  • rotavirus

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

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