TY - JOUR
T1 - Intracellular neuronal recording in awake nonhuman primates
AU - Gao, Lixia
AU - Wang, Xiaoqin
N1 - Funding Information:
We thank Xinjian Li and Lingyun Zhao at Johns Hopkins University (JHU) for writing the Matlab analysis code, Shiyong Huang and Alfredo Kirkwood at JHU for providing access to the pipette puller (model P-2000), Anna Wang Roe at ZIINT for critically reading and commenting on the manuscript. We thank previous members of the Wang Laboratory at JHU who made contributions to the development of the awake and behaving marmoset preparation on which the present study was based. This work was supported by National Institutes of Health grants DC003180, DC005808 and DC014503.
Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Intracellular neuronal recordings from the brain of awake nonhuman primates have remained difficult to obtain because of several formidable technical challenges, such as poor recording stability and difficulties in maintaining long-term recording conditions. We have developed a technique to record neuronal activity by using a coaxial guide tube and sharp electrode assembly, which allows researchers to repeatedly and reliably perform intracellular recordings in the cortex of awake marmosets. Recordings from individual neurons last from several minutes to more than an hour. A key advantage of this approach is that it does not require dura removal, permitting recordings over weeks and months in a single animal. This protocol describes the step-by-step procedures for construction of a custom-made marmoset chair, head-cap implantation, preparation of the sharp electrode and guide tube, neuronal recording and data analysis. As the technique is practical and easy to adapt, we anticipate that it can also be applied to other mammalian models, including larger-size nonhuman primates.
AB - Intracellular neuronal recordings from the brain of awake nonhuman primates have remained difficult to obtain because of several formidable technical challenges, such as poor recording stability and difficulties in maintaining long-term recording conditions. We have developed a technique to record neuronal activity by using a coaxial guide tube and sharp electrode assembly, which allows researchers to repeatedly and reliably perform intracellular recordings in the cortex of awake marmosets. Recordings from individual neurons last from several minutes to more than an hour. A key advantage of this approach is that it does not require dura removal, permitting recordings over weeks and months in a single animal. This protocol describes the step-by-step procedures for construction of a custom-made marmoset chair, head-cap implantation, preparation of the sharp electrode and guide tube, neuronal recording and data analysis. As the technique is practical and easy to adapt, we anticipate that it can also be applied to other mammalian models, including larger-size nonhuman primates.
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U2 - 10.1038/s41596-020-0388-3
DO - 10.1038/s41596-020-0388-3
M3 - Article
C2 - 33046899
AN - SCOPUS:85092413637
SN - 1754-2189
VL - 15
SP - 3615
EP - 3631
JO - Nature protocols
JF - Nature protocols
IS - 11
ER -