Intracellular metallothionein concentration and the rate of zinc or cadmium influx and MT mRNA accumulation in a CHO Cdr variant

Sheldon Morris; P. C. Huang

doi:10.1016/0014-4827(89)90046-3

Intracellular metallothionein concentration and the rate of zinc or cadmium influx and MT mRNA accumulation in a CHO Cd^r variant

Sheldon Morris, P. C. Huang

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The regulation of metallothionein (MT) gene expression in a cadmium resistant CHO cell line which overproduces MT was examined in this study. Our results show that MT mRNA levels reach a maximum 24-30 h after a primary zinc exposure and, subsequently, MT mRNA concentrations decline. This decrease in MT mRNA levels can be correlated with the accumulation of metallothionein and decreased rates of zinc and cadmium uptake.

Original language	English (US)
Pages (from-to)	166-175
Number of pages	10
Journal	Experimental cell research
Volume	185
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(89)90046-3
State	Published - Nov 1989

ASJC Scopus subject areas

Cell Biology

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10.1016/0014-4827(89)90046-3

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title = "Intracellular metallothionein concentration and the rate of zinc or cadmium influx and MT mRNA accumulation in a CHO Cdr variant",

abstract = "The regulation of metallothionein (MT) gene expression in a cadmium resistant CHO cell line which overproduces MT was examined in this study. Our results show that MT mRNA levels reach a maximum 24-30 h after a primary zinc exposure and, subsequently, MT mRNA concentrations decline. This decrease in MT mRNA levels can be correlated with the accumulation of metallothionein and decreased rates of zinc and cadmium uptake.",

author = "Sheldon Morris and Huang, {P. C.}",

note = "Funding Information: Supportedi n part by USPHS NIH Grant ROl GM32606.W e thank Ms. I. P. Tseng for assistancei n repeatingt he measurementos f MT mRNA and MT and Mrs. Betty Chinn Smith for assistancei n the preparationo f this manuscript.",

year = "1989",

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doi = "10.1016/0014-4827(89)90046-3",

language = "English (US)",

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journal = "Experimental Cell Research",

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T1 - Intracellular metallothionein concentration and the rate of zinc or cadmium influx and MT mRNA accumulation in a CHO Cdr variant

AU - Morris, Sheldon

AU - Huang, P. C.

N1 - Funding Information: Supportedi n part by USPHS NIH Grant ROl GM32606.W e thank Ms. I. P. Tseng for assistancei n repeatingt he measurementos f MT mRNA and MT and Mrs. Betty Chinn Smith for assistancei n the preparationo f this manuscript.

PY - 1989/11

Y1 - 1989/11

N2 - The regulation of metallothionein (MT) gene expression in a cadmium resistant CHO cell line which overproduces MT was examined in this study. Our results show that MT mRNA levels reach a maximum 24-30 h after a primary zinc exposure and, subsequently, MT mRNA concentrations decline. This decrease in MT mRNA levels can be correlated with the accumulation of metallothionein and decreased rates of zinc and cadmium uptake.

AB - The regulation of metallothionein (MT) gene expression in a cadmium resistant CHO cell line which overproduces MT was examined in this study. Our results show that MT mRNA levels reach a maximum 24-30 h after a primary zinc exposure and, subsequently, MT mRNA concentrations decline. This decrease in MT mRNA levels can be correlated with the accumulation of metallothionein and decreased rates of zinc and cadmium uptake.

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U2 - 10.1016/0014-4827(89)90046-3

DO - 10.1016/0014-4827(89)90046-3

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JO - Experimental Cell Research

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ER -

Intracellular metallothionein concentration and the rate of zinc or cadmium influx and MT mRNA accumulation in a CHO Cd^r variant

Abstract

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