Intracellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein: Absence of nucleolar accumulation during infection and after expression as a recombinant protein in Vero cells

Raymond R.R. Rowland, Vinita Chauhan, Ying Fang, Andrew Pekosz, Maureen Kerrigan, Miriam D. Burton

Research output: Contribution to journalArticlepeer-review

Abstract

The nucleocapsid (N) protein of several members within the order Nidovirales localizes to the nucleolus during infection and after transfection of cells with N genes. However, confocal microscopy of N protein localization in Vero cells infected with the severe acute respiratory syndrome coronavirus (SARS-CoV) or transfected with the SARS-CoV N gene failed to show the presence of N in the nucleoplasm or nucleolus. Amino acids 369 to 389, which contain putative nuclear localization signal (NLS) and nucleolar localization signal motifs, failed to restore nuclear localization to an NLS-minus mutant Rev protein. These data indicate that nuclear localization is not a conserved property among all nidoviruses.

Original languageEnglish (US)
Pages (from-to)11507-11512
Number of pages6
JournalJournal of virology
Volume79
Issue number17
DOIs
StatePublished - Sep 2005
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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