Abstract
Objective: To assess if the reduction in HIV-1 RNA in CD4+ T cells is correlated with the persistence of immune activation following early antiretroviral therapy (ART). Design: Clinical trial (NCT01285050). Methods: Next-generation sequencing was used to study total RNA from activated CD4+ T cells (CD38 and human leukocyte antigen - antigen D related (HLA-DR) expressing) collected from 19 treatment-naïve HIV-1/hepatitis C virus-infected patients before and early after ART initiation (≥12 weeks after plasma HIV-1 RNA <50 copies/ml). To validate comparisons, pre and post-ART measures were adjusted for input RNA and overall read number. Results: As expected, ART use was associated with a median [interquartile range (IQR)] 4.3% (2.2-8.3) reduction in the proportion of activated CD4+ T cells (P = 0.0008). Whereas in those activated CD4+ T cells no consistent differences in overall gene expression were detected, interferon-stimulated gene expression declined (P < 2 × 10-16). Pre-ART, sorted activated CD4+ T cells contained a median (IQR) of 959 (252-1614) HIV-1 reads/107 reads compared with 72 (55-152) HIV-1 reads/107 reads after at least 12 weeks of suppressive ART (P = 8 × 10-5). The decrease in HIV-1 reads in activated CD4+ T cells was associated with the change in plasma HIV-1 RNA levels (r = 0.77, P = 2 × 10-4) and the change in the proportion of activated CD4+ T cells (r = 0.70, P = 0.0016). Conclusion: Months of ART led to a marked decrease in cell-associated HIV-1 RNA and interferon-stimulated genes expression in activated CD4+ T cells that were strongly associated with the reduction in the proportion of activated CD4+ T cells.
Original language | English (US) |
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Pages (from-to) | 1405-1414 |
Number of pages | 10 |
Journal | AIDS |
Volume | 31 |
Issue number | 10 |
DOIs | |
State | Published - Jun 19 2017 |
Keywords
- HIV-1
- Immune activation
- T-cell activation
- antiretroviral therapy
- cell-associated HIV-1 RNA
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Infectious Diseases