Intracellular expression of reactive oxygen species-generating NADPH oxidase NOX4 in normal and cancer thyroid tissues

Sossou Weyemi, Bernard Caillou, Monique Talbot, Rabii Ameziane-El-Hassani, Ludovic Lacroix, Odile Lagent-Chevallier, Abir Al Ghuzlan, Dirk Roos, Jean Michel Bidart, Alain Virion, Martin Schlumberger, Corinne Dupuy

Research output: Contribution to journalArticle

Abstract

NADPH oxidase 4 (NOX4) belongs to the NOX family that generates reactive oxygen species (ROS). Function and tissue distribution of NOX4 have not yet been entirely clarified. To date, in the thyroid gland, only DUOX1/2 NOX systems have been described. NOX4 mRNA expression, as shown by real-time PCR, was present in normal thyroid tissue, regulated by TSH and significantly increased in differentiated cancer tissues. TSH increased the protein level of NOX4 in human thyroid primary culture and NOX4-dependent ROS generation. NOX4 immunostaining was detected in normal and pathologic thyroid tissues. In normal thyroid tissue, staining was heterogeneous and mostly found in activated columnar thyrocytes but absent in quiescent flat cells. Papillary and follicular thyroid carcinomas displayed more homogeneous staining. The p22phox protein that forms a heterodimeric enzyme complex with NOX4 displayed an identical cellular expression pattern and was also positively regulated by TSH. ROS may have various biological effects, depending on the site of production. Intracellular NOX4-p22phox localization suggests a role in cytoplasmic redox signaling, in contrast to the DUOX localization at the apical membrane that corresponds to an extracellular H2O2 production. Increased NOX4-p22phox in cancer might be related to a higher proliferation rate and tumor progression but a role in the development of tumors has to be further studied and established in the future.

Original languageEnglish (US)
Pages (from-to)27-37
Number of pages11
JournalEndocrine-Related Cancer
Volume17
Issue number1
DOIs
StatePublished - Mar 2010
Externally publishedYes

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NADPH Oxidase
Thyroid Neoplasms
Reactive Oxygen Species
Thyroid Gland
Neoplasms
Follicular Adenocarcinoma
Staining and Labeling
Tissue Distribution
Oxidation-Reduction
Real-Time Polymerase Chain Reaction
Proteins
Messenger RNA
Membranes
Enzymes

ASJC Scopus subject areas

  • Endocrinology
  • Oncology
  • Cancer Research
  • Endocrinology, Diabetes and Metabolism

Cite this

Weyemi, S., Caillou, B., Talbot, M., Ameziane-El-Hassani, R., Lacroix, L., Lagent-Chevallier, O., ... Dupuy, C. (2010). Intracellular expression of reactive oxygen species-generating NADPH oxidase NOX4 in normal and cancer thyroid tissues. Endocrine-Related Cancer, 17(1), 27-37. https://doi.org/10.1677/ERC-09-0175

Intracellular expression of reactive oxygen species-generating NADPH oxidase NOX4 in normal and cancer thyroid tissues. / Weyemi, Sossou; Caillou, Bernard; Talbot, Monique; Ameziane-El-Hassani, Rabii; Lacroix, Ludovic; Lagent-Chevallier, Odile; Al Ghuzlan, Abir; Roos, Dirk; Bidart, Jean Michel; Virion, Alain; Schlumberger, Martin; Dupuy, Corinne.

In: Endocrine-Related Cancer, Vol. 17, No. 1, 03.2010, p. 27-37.

Research output: Contribution to journalArticle

Weyemi, S, Caillou, B, Talbot, M, Ameziane-El-Hassani, R, Lacroix, L, Lagent-Chevallier, O, Al Ghuzlan, A, Roos, D, Bidart, JM, Virion, A, Schlumberger, M & Dupuy, C 2010, 'Intracellular expression of reactive oxygen species-generating NADPH oxidase NOX4 in normal and cancer thyroid tissues', Endocrine-Related Cancer, vol. 17, no. 1, pp. 27-37. https://doi.org/10.1677/ERC-09-0175
Weyemi, Sossou ; Caillou, Bernard ; Talbot, Monique ; Ameziane-El-Hassani, Rabii ; Lacroix, Ludovic ; Lagent-Chevallier, Odile ; Al Ghuzlan, Abir ; Roos, Dirk ; Bidart, Jean Michel ; Virion, Alain ; Schlumberger, Martin ; Dupuy, Corinne. / Intracellular expression of reactive oxygen species-generating NADPH oxidase NOX4 in normal and cancer thyroid tissues. In: Endocrine-Related Cancer. 2010 ; Vol. 17, No. 1. pp. 27-37.
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