TY - JOUR
T1 - Intracellular endosomal magnetic labeling of cells.
AU - Bulte, Jeff W.M.
PY - 2006
Y1 - 2006
N2 - Cellular imaging encompasses the noninvasive and repetitive imaging of targeted cells and cellular processes in living organisms. Magnetic resonance imaging (MRI), with its excellent spatial resolution, is ideally suited to provide unique information on the location and migration of cells after transplantation or transfusion. This approach requires magnetic prelabeling of the cells of choice. In this chapter, several methods and techniques will be described that can be applied for an efficient intracellular magnetic labeling of cells. In addition, a few basic protocols for the analysis and evaluation of cell labeling will be provided. The chapter will focus on the use of superparamagnetic iron oxides, because they are biocompatible and have strong effects on T2* relaxation. With the currently available magnetic labeling methods, it is anticipated that cellular MRI will find broad applications in biology and medicine.
AB - Cellular imaging encompasses the noninvasive and repetitive imaging of targeted cells and cellular processes in living organisms. Magnetic resonance imaging (MRI), with its excellent spatial resolution, is ideally suited to provide unique information on the location and migration of cells after transplantation or transfusion. This approach requires magnetic prelabeling of the cells of choice. In this chapter, several methods and techniques will be described that can be applied for an efficient intracellular magnetic labeling of cells. In addition, a few basic protocols for the analysis and evaluation of cell labeling will be provided. The chapter will focus on the use of superparamagnetic iron oxides, because they are biocompatible and have strong effects on T2* relaxation. With the currently available magnetic labeling methods, it is anticipated that cellular MRI will find broad applications in biology and medicine.
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U2 - 10.1385/1-59745-010-3:419
DO - 10.1385/1-59745-010-3:419
M3 - Review article
C2 - 16506433
AN - SCOPUS:33645232205
SN - 1543-1894
VL - 124
SP - 419
EP - 439
JO - Methods in Molecular Medicine
JF - Methods in Molecular Medicine
ER -