Intracellular calcium transients and developed tension in rat heart muscle: A mechanismfor the negative interval-strength relationship

C. H. Orchard, Edward Lakatta

Research output: Contribution to journalArticle

Abstract

Thepurposes of the present study were to determine (a) whether changes of intracellular [Ca2+] (Ca;) can account for the decrease of developed tension observed in rat heart muscle when stimulation rate is increased, and (6) whether the effect of stimulation rate on Ca; is altered in conditions in which the rate of repriming of the sarcoplasmic reticulum (SR) is altered, as when perfusate [Ca2+] (Ca0) is increased, and in heart muscle from senescent animals. The photoprotein aequorin was used to monitor Cai in rat papillary muscles. In muscles from 6-mo-old rats, increasing the stimulation rate in the range 0.2-0.66 Hz led to parallel decreases of both the aequorin light transient and developed tension when Cao was 2 mM. When Ca0 was increased to 4 mM, changes in the stimulation rate had less effect on both the light transient and tension. At 8 mM Ca0 changing the stimulation rate had no effect on either the light transient or developed tension. Papillary muscles from 24-mo-old rats, in which SR function is likely to be depressed, exhibited a prolonged Ca21 transient and twitch. At a Ca of 4 or 8 mM, increasing the stimulation rate from 0.33 to 0.66 Hz still led to decreases in the size of the aequorin light transient and developed tension in these muscles. Developed tension and aequorin light responded to increases of Ca0 in the same way in both groups of muscles. We conclude that under the conditions of our experiments, developed tension is determined by Ca0. The negative interval-strength relationship observed when Ca0 is in the physiological range can be accounted for by a timedependent recycling of Ca2+ by the SR. The effects of increasing Ca0 and the age-related differences observed at high Ca0 can also be accounted for using this model.

Original languageEnglish (US)
Pages (from-to)637-651
Number of pages15
JournalJournal of General Physiology
Volume86
Issue number5
DOIs
StatePublished - Nov 1 1985
Externally publishedYes

Fingerprint

Aequorin
Myocardium
Calcium
Light
Sarcoplasmic Reticulum
Papillary Muscles
Luminescent Proteins
Muscles
Muscle Tonus
Recycling

ASJC Scopus subject areas

  • Physiology

Cite this

@article{34854bb6e2ff4334ae7938e63d62a9c0,
title = "Intracellular calcium transients and developed tension in rat heart muscle: A mechanismfor the negative interval-strength relationship",
abstract = "Thepurposes of the present study were to determine (a) whether changes of intracellular [Ca2+] (Ca;) can account for the decrease of developed tension observed in rat heart muscle when stimulation rate is increased, and (6) whether the effect of stimulation rate on Ca; is altered in conditions in which the rate of repriming of the sarcoplasmic reticulum (SR) is altered, as when perfusate [Ca2+] (Ca0) is increased, and in heart muscle from senescent animals. The photoprotein aequorin was used to monitor Cai in rat papillary muscles. In muscles from 6-mo-old rats, increasing the stimulation rate in the range 0.2-0.66 Hz led to parallel decreases of both the aequorin light transient and developed tension when Cao was 2 mM. When Ca0 was increased to 4 mM, changes in the stimulation rate had less effect on both the light transient and tension. At 8 mM Ca0 changing the stimulation rate had no effect on either the light transient or developed tension. Papillary muscles from 24-mo-old rats, in which SR function is likely to be depressed, exhibited a prolonged Ca21 transient and twitch. At a Ca of 4 or 8 mM, increasing the stimulation rate from 0.33 to 0.66 Hz still led to decreases in the size of the aequorin light transient and developed tension in these muscles. Developed tension and aequorin light responded to increases of Ca0 in the same way in both groups of muscles. We conclude that under the conditions of our experiments, developed tension is determined by Ca0. The negative interval-strength relationship observed when Ca0 is in the physiological range can be accounted for by a timedependent recycling of Ca2+ by the SR. The effects of increasing Ca0 and the age-related differences observed at high Ca0 can also be accounted for using this model.",
author = "Orchard, {C. H.} and Edward Lakatta",
year = "1985",
month = "11",
day = "1",
doi = "10.1085/jgp.86.5.637",
language = "English (US)",
volume = "86",
pages = "637--651",
journal = "Journal of General Physiology",
issn = "0022-1295",
publisher = "Rockefeller University Press",
number = "5",

}

TY - JOUR

T1 - Intracellular calcium transients and developed tension in rat heart muscle

T2 - A mechanismfor the negative interval-strength relationship

AU - Orchard, C. H.

AU - Lakatta, Edward

PY - 1985/11/1

Y1 - 1985/11/1

N2 - Thepurposes of the present study were to determine (a) whether changes of intracellular [Ca2+] (Ca;) can account for the decrease of developed tension observed in rat heart muscle when stimulation rate is increased, and (6) whether the effect of stimulation rate on Ca; is altered in conditions in which the rate of repriming of the sarcoplasmic reticulum (SR) is altered, as when perfusate [Ca2+] (Ca0) is increased, and in heart muscle from senescent animals. The photoprotein aequorin was used to monitor Cai in rat papillary muscles. In muscles from 6-mo-old rats, increasing the stimulation rate in the range 0.2-0.66 Hz led to parallel decreases of both the aequorin light transient and developed tension when Cao was 2 mM. When Ca0 was increased to 4 mM, changes in the stimulation rate had less effect on both the light transient and tension. At 8 mM Ca0 changing the stimulation rate had no effect on either the light transient or developed tension. Papillary muscles from 24-mo-old rats, in which SR function is likely to be depressed, exhibited a prolonged Ca21 transient and twitch. At a Ca of 4 or 8 mM, increasing the stimulation rate from 0.33 to 0.66 Hz still led to decreases in the size of the aequorin light transient and developed tension in these muscles. Developed tension and aequorin light responded to increases of Ca0 in the same way in both groups of muscles. We conclude that under the conditions of our experiments, developed tension is determined by Ca0. The negative interval-strength relationship observed when Ca0 is in the physiological range can be accounted for by a timedependent recycling of Ca2+ by the SR. The effects of increasing Ca0 and the age-related differences observed at high Ca0 can also be accounted for using this model.

AB - Thepurposes of the present study were to determine (a) whether changes of intracellular [Ca2+] (Ca;) can account for the decrease of developed tension observed in rat heart muscle when stimulation rate is increased, and (6) whether the effect of stimulation rate on Ca; is altered in conditions in which the rate of repriming of the sarcoplasmic reticulum (SR) is altered, as when perfusate [Ca2+] (Ca0) is increased, and in heart muscle from senescent animals. The photoprotein aequorin was used to monitor Cai in rat papillary muscles. In muscles from 6-mo-old rats, increasing the stimulation rate in the range 0.2-0.66 Hz led to parallel decreases of both the aequorin light transient and developed tension when Cao was 2 mM. When Ca0 was increased to 4 mM, changes in the stimulation rate had less effect on both the light transient and tension. At 8 mM Ca0 changing the stimulation rate had no effect on either the light transient or developed tension. Papillary muscles from 24-mo-old rats, in which SR function is likely to be depressed, exhibited a prolonged Ca21 transient and twitch. At a Ca of 4 or 8 mM, increasing the stimulation rate from 0.33 to 0.66 Hz still led to decreases in the size of the aequorin light transient and developed tension in these muscles. Developed tension and aequorin light responded to increases of Ca0 in the same way in both groups of muscles. We conclude that under the conditions of our experiments, developed tension is determined by Ca0. The negative interval-strength relationship observed when Ca0 is in the physiological range can be accounted for by a timedependent recycling of Ca2+ by the SR. The effects of increasing Ca0 and the age-related differences observed at high Ca0 can also be accounted for using this model.

UR - http://www.scopus.com/inward/record.url?scp=0022387848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022387848&partnerID=8YFLogxK

U2 - 10.1085/jgp.86.5.637

DO - 10.1085/jgp.86.5.637

M3 - Article

C2 - 4067571

AN - SCOPUS:0022387848

VL - 86

SP - 637

EP - 651

JO - Journal of General Physiology

JF - Journal of General Physiology

SN - 0022-1295

IS - 5

ER -