Intraarterial adenosine administered during reperfusion preserves endothelium-dependent relaxation in the rabbit hindlimb

Michael M. Farooq, Julie A. Freischlag, Brian T. Carlsen, Robert A. Cambria, Gary R. Seabrook, Jonathan B. Towne

Research output: Contribution to journalArticle

Abstract

Consumption of adenosine triphosphate (ATP) during ischemia leads to the failure of cell membrane ATP-dependent ion pumps, cell lysis, and activation of the inflammatory cascade, which contributes to endothelial injury during reperfusion. Adenosine is a lipid-soluble precursor in ATP metabolism that may be depleted during reperfusion, exacerbating this injury. The purpose of this study was to examine the effect of adenosine administration with reperfusion on endothelium-dependent relaxation in the rabbit superficial femoral artery. New Zealand white rabbits underwent 3 hours of complete unilateral hindlimb ischemia followed by 2 hours of reperfusion. Animals received an intraarterial infusion of adenosine (0.35 mg/kg/min) or an equivalent volume of normal saline (1 cc/min) during the first 20 minutes of reperfusion. A sham group underwent aortoiliac collateral ligation and 5 hours of anesthesia without ischemia. A control group underwent immediate superficial femoral artery explantation with no collateral ligation or prolonged anesthesia. The superficial femoral arteries were explanted and suspended as 3 mm segments upon isometric force transducers within organ bath chambers. Contraction was evaluated with potassium chloride (KCl) and maximal norepinephrine (NE). Acetylcholine (Ach) relaxation was determined after NE submaximal contraction. No difference in contractile response to KCl was observed among the four groups. A significant increase in contraction to maximum NE was observed for vessels in the adenosine group (P

Original languageEnglish (US)
Pages (from-to)713-719
Number of pages7
JournalVascular Surgery
Volume31
Issue number6
StatePublished - 1997
Externally publishedYes

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Hindlimb
Adenosine
Reperfusion
Endothelium
Femoral Artery
Rabbits
Norepinephrine
Ischemia
Adenosine Triphosphate
Reperfusion Injury
Ligation
Anesthesia
Ion Pumps
Intra Arterial Infusions
Potassium Chloride
Transducers
Baths
Acetylcholine
Cell Membrane
Lipids

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Farooq, M. M., Freischlag, J. A., Carlsen, B. T., Cambria, R. A., Seabrook, G. R., & Towne, J. B. (1997). Intraarterial adenosine administered during reperfusion preserves endothelium-dependent relaxation in the rabbit hindlimb. Vascular Surgery, 31(6), 713-719.

Intraarterial adenosine administered during reperfusion preserves endothelium-dependent relaxation in the rabbit hindlimb. / Farooq, Michael M.; Freischlag, Julie A.; Carlsen, Brian T.; Cambria, Robert A.; Seabrook, Gary R.; Towne, Jonathan B.

In: Vascular Surgery, Vol. 31, No. 6, 1997, p. 713-719.

Research output: Contribution to journalArticle

Farooq, MM, Freischlag, JA, Carlsen, BT, Cambria, RA, Seabrook, GR & Towne, JB 1997, 'Intraarterial adenosine administered during reperfusion preserves endothelium-dependent relaxation in the rabbit hindlimb', Vascular Surgery, vol. 31, no. 6, pp. 713-719.
Farooq MM, Freischlag JA, Carlsen BT, Cambria RA, Seabrook GR, Towne JB. Intraarterial adenosine administered during reperfusion preserves endothelium-dependent relaxation in the rabbit hindlimb. Vascular Surgery. 1997;31(6):713-719.
Farooq, Michael M. ; Freischlag, Julie A. ; Carlsen, Brian T. ; Cambria, Robert A. ; Seabrook, Gary R. ; Towne, Jonathan B. / Intraarterial adenosine administered during reperfusion preserves endothelium-dependent relaxation in the rabbit hindlimb. In: Vascular Surgery. 1997 ; Vol. 31, No. 6. pp. 713-719.
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