Intra-articular Injection of Urinary Bladder Matrix Reduces Osteoarthritis Development

Heather N. Jacobs, Sona Rathod, Matthew T. Wolf, Jennifer H. Elisseeff

Research output: Contribution to journalArticle

Abstract

Micronized porcine urinary bladder matrix (UBM) is an extracellular matrix biomaterial that has immunomodulatory and pro-regenerative properties. The objective of this study was to assess the ability of UBM to alter disease progression in a mouse model of post-traumatic osteoarthritis (OA). Ten-week-old wild-type C57BL/6 male mice underwent anterior cruciate ligament transection (ACLT) to induce OA. Two weeks after ACLT, UBM (50 mg/mL) or saline was injected into the mouse joint. At 4 and 8 weeks post-ACLT, cartilage integrity was assessed using OARSI scoring of histology, pain was evaluated, and joints were harvested for quantitative RT-PCR analysis of cartilage-specific and inflammatory gene expression. UBM-treated animals showed improved cartilage integrity at 4 and 8 weeks and reduced pain at 4 weeks compared to saline-injected mice. Animals injected with UBM expressed higher levels of genes encoding structural cartilage proteins, such as collagen2α1 and aggrecan, as well as anti-inflammatory cytokines, including interleukins 10 and 4. UBM decreased cartilage degeneration in the murine ACLT model of OA, which may be due to reduced inflammation in the joint and maintenance of high expression levels of proteoglycans.

Original languageEnglish (US)
Pages (from-to)141-149
Number of pages9
JournalAAPS Journal
Volume19
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Intra-Articular Injections
Osteoarthritis
Urinary Bladder
Anterior Cruciate Ligament
Cartilage
Joints
Aggrecans
Pain
Aptitude
Biocompatible Materials
Proteoglycans
Interleukin-4
Interleukin-10
Extracellular Matrix
Disease Progression
Histology
Anti-Inflammatory Agents
Swine
Cytokines
Inflammation

Keywords

  • ACL transection
  • bioengineering
  • extracellular matrix
  • osteoarthritis
  • urinary bladder matrix

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Intra-articular Injection of Urinary Bladder Matrix Reduces Osteoarthritis Development. / Jacobs, Heather N.; Rathod, Sona; Wolf, Matthew T.; Elisseeff, Jennifer H.

In: AAPS Journal, Vol. 19, No. 1, 01.01.2017, p. 141-149.

Research output: Contribution to journalArticle

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