Intimate associations between the endogenous opiate systems and the growth hormone-releasing hormone system in the human hypothalamus

J. Olsen, M. Peroski, Matthew Kiczek, G. Grignol, I. Merchenthaler, B. Dudas

Research output: Contribution to journalArticle

Abstract

Although it is a general consensus that opioids modulate growth, the mechanism of this phenomenon is largely unknown. Since endogenous opiates use the same receptor family as morphine, these peptides may be one of the key regulators of growth in humans by impacting growth hormone (GH) secretion, either directly, or indirectly, via growth hormone-releasing hormone (GHRH) release. However, the exact mechanism of this regulation has not been elucidated yet.In the present study we identified close juxtapositions between the enkephalinergic/endorphinergic/dynorphinergic axonal varicosities and GHRH-immunoreactive (IR) perikarya in the human hypothalamus. Due to the long post mortem period electron microscopy could not be utilized to detect the presence of synapses between the enkephalinergic/endorphinergic/dynorphinergic and GHRH neurons. Therefore, we used light microscopic double-label immunocytochemistry to identify putative juxtapositions between these systems.Our findings revealed that the majority of the GHRH-IR perikarya formed intimate associations with enkephalinergic axonal varicosities in the infundibular nucleus/median eminence, while endorphinergic-GHRH juxtapositions were much less frequent. In contrast, no significant dynorphinergic-GHRH associations were detected.The density of the abutting enkephalinergic fibers on the surface of the GHRH perikarya suggests that these juxtapositions may be functional synapses and may represent the morphological substrate of the impact of enkephalin on growth. The small number of GHRH neurons innervated by the endorphin and dynorphin systems indicates significant differences between the regulatory roles of endogenous opiates on growth in humans.

Original languageEnglish (US)
Pages (from-to)238-245
Number of pages8
JournalNeuroscience
Volume258
DOIs
StatePublished - Jan 31 2014
Externally publishedYes

Fingerprint

Growth Hormone-Releasing Hormone
Opioid Peptides
Hypothalamus
Growth
Synapses
Endorphins
Neurons
Dynorphins
Median Eminence
Arcuate Nucleus of Hypothalamus
Human Growth Hormone
Enkephalins
Morphine
Opioid Analgesics
Electron Microscopy
Immunohistochemistry
Light
Peptides

Keywords

  • Dynorphin
  • Endorphin
  • Enkephalin
  • GHRH
  • Growth
  • Hypothalamus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Intimate associations between the endogenous opiate systems and the growth hormone-releasing hormone system in the human hypothalamus. / Olsen, J.; Peroski, M.; Kiczek, Matthew; Grignol, G.; Merchenthaler, I.; Dudas, B.

In: Neuroscience, Vol. 258, 31.01.2014, p. 238-245.

Research output: Contribution to journalArticle

Olsen, J. ; Peroski, M. ; Kiczek, Matthew ; Grignol, G. ; Merchenthaler, I. ; Dudas, B. / Intimate associations between the endogenous opiate systems and the growth hormone-releasing hormone system in the human hypothalamus. In: Neuroscience. 2014 ; Vol. 258. pp. 238-245.
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AU - Dudas, B.

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