Intestinal stem cell growth and differentiation on a tubular scaffold with evaluation in small and large animals

Shahab A. Shaffiey, Hongpeng Jia, Timothy Keane, Cait Costello, Deena Wasserman, Maria Quidgley, Jenna Dziki, Stephen Badylak, Chhinder P. Sodhi, John C. March, David J. Hackam

Research output: Contribution to journalArticle

Abstract

Aims: To investigate the growth and differentiation of intestinal stem cells on a novel tubular scaffold in vitro and in vivo. Materials & methods: Intestinal progenitor cells from mice or humans were cultured with myofibroblasts, macrophages and/or bacteria, and evaluated in mice via omental implantation. Mucosal regeneration was evaluated in dogs after rectal mucosectomy followed by scaffold implantation. Results: Intestinal progenitor cells differentiated into crypt-villi structures on the scaffold. Differentiation and scaffold coverage was enhanced by coculture with myofibroblasts, macrophages and probiotic bacteria, while the implanted scaffolds enhanced mucosal regeneration in the dog rectum. Conclusion: Intestinal stem cell growth and differentiation on a novel tubular scaffold is enhanced through addition of cellular and microbial components, as validated in mice and dogs.

Original languageEnglish (US)
Pages (from-to)45-61
Number of pages17
JournalRegenerative Medicine
Volume11
Issue number1
DOIs
StatePublished - Jan 2016

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Keywords

  • artificial intestine
  • intestinal stem cells
  • necrotizing enterocolitis
  • scaffold
  • short bowel syndrome
  • tissue-engineered small intestine

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

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