TY - JOUR
T1 - Intestinal electric stimulation modulates neuronal activity in the nucleus of the solitary tract in rats
AU - Sun, Ying
AU - Qin, Chao
AU - Foreman, Robert D.
AU - Chen, J. D.Z.
N1 - Funding Information:
The authors thank D. Holston for expert technical assistance. This work was supported by a grant from National Institutes of Health (1R43-DK063733-01).
PY - 2005/9/2
Y1 - 2005/9/2
N2 - Intestinal electric stimulation (IES) has been shown to produce an inhibitory effect on gastric motility and secretion. The possible central mechanism of this entero-gastric inhibitory effect induced by IES is unknown. The objective of this study was to evaluate the effects of various IES on the activity of neurons in nucleus tractus solitarii (NTS). We examined the extracellular neuronal activity in NTS of the medulla in pentobarbital anesthetized, paralyzed, ventilated male adult rats. The aortic depressor, superior laryngeal, and carotid sinus nerves were crushed or sectioned bilaterally to avoid neuronal responses in NTS to cardiovascular baroreceptors. After NTS neurons with gastric input were identified, responses of single neurons in NTS to IES were determined. IES with different parameters was performed via a pair of platinum electrodes sutured onto the serosal surface of the duodenum 2 cm below the pylorus. IES with different parameters activated 39-72% of the solitary tract nucleus neurons responsive to gastric distension. Moreover, we demonstrated that IES activated the neuronal activity in NTS, which was stimulation energy dependent. The modulatory effect of IES on the central neurons receiving vagal inputs may contribute to the neural mechanisms of IES therapy for the treatment of patients with obesity and gastrointestinal motility disorders.
AB - Intestinal electric stimulation (IES) has been shown to produce an inhibitory effect on gastric motility and secretion. The possible central mechanism of this entero-gastric inhibitory effect induced by IES is unknown. The objective of this study was to evaluate the effects of various IES on the activity of neurons in nucleus tractus solitarii (NTS). We examined the extracellular neuronal activity in NTS of the medulla in pentobarbital anesthetized, paralyzed, ventilated male adult rats. The aortic depressor, superior laryngeal, and carotid sinus nerves were crushed or sectioned bilaterally to avoid neuronal responses in NTS to cardiovascular baroreceptors. After NTS neurons with gastric input were identified, responses of single neurons in NTS to IES were determined. IES with different parameters was performed via a pair of platinum electrodes sutured onto the serosal surface of the duodenum 2 cm below the pylorus. IES with different parameters activated 39-72% of the solitary tract nucleus neurons responsive to gastric distension. Moreover, we demonstrated that IES activated the neuronal activity in NTS, which was stimulation energy dependent. The modulatory effect of IES on the central neurons receiving vagal inputs may contribute to the neural mechanisms of IES therapy for the treatment of patients with obesity and gastrointestinal motility disorders.
KW - Entero-gastric inhibitory reflex
KW - Gastrointestinal motility disorders
KW - Intestinal electric stimulation
KW - Nucleus tractus solitarii
KW - Obesity
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U2 - 10.1016/j.neulet.2005.05.008
DO - 10.1016/j.neulet.2005.05.008
M3 - Article
C2 - 15951110
AN - SCOPUS:21344450505
SN - 0304-3940
VL - 385
SP - 64
EP - 69
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -