Intersectional monosynaptic tracing for dissecting subtype-specific organization of GABAergic interneuron inputs

Michael J. Yetman, Eric Washburn, Jung Ho Hyun, Fumitaka Osakada, Yasufumi Hayano, Hongkui Zeng, Edward M. Callaway, Hyungbae Kwon, Hiroki Taniguchi

Research output: Contribution to journalArticle

Abstract

Functionally and anatomically distinct cortical substructures, such as areas or layers, contain different principal neuron (PN) subtypes that generate output signals representing particular information. Various types of cortical inhibitory interneurons (INs) differentially but coordinately regulate PN activity. Despite a potential determinant for functional specialization of PN subtypes, the spatial organization of IN subtypes that innervate defined PN subtypes remains unknown. Here we develop a genetic strategy combining a recombinase-based intersectional labeling method and rabies viral monosynaptic tracing, which enables subtype-specific visualization of cortical IN ensembles sending inputs to defined PN subtypes. Our approach reveals not only cardinal but also underrepresented connections between broad, non-overlapping IN subtypes and PNs. Furthermore, we demonstrate that distinct PN subtypes defined by areal or laminar positions display different organization of input IN subtypes. Our genetic strategy will facilitate understanding of the wiring and developmental principles of cortical inhibitory circuits at unparalleled levels.

Original languageEnglish (US)
Pages (from-to)492-502
Number of pages11
JournalNature neuroscience
Volume22
Issue number3
DOIs
StatePublished - Mar 1 2019
Externally publishedYes

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Interneurons
Neurons
Recombinases
Rabies

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Yetman, M. J., Washburn, E., Hyun, J. H., Osakada, F., Hayano, Y., Zeng, H., ... Taniguchi, H. (2019). Intersectional monosynaptic tracing for dissecting subtype-specific organization of GABAergic interneuron inputs. Nature neuroscience, 22(3), 492-502. https://doi.org/10.1038/s41593-018-0322-y

Intersectional monosynaptic tracing for dissecting subtype-specific organization of GABAergic interneuron inputs. / Yetman, Michael J.; Washburn, Eric; Hyun, Jung Ho; Osakada, Fumitaka; Hayano, Yasufumi; Zeng, Hongkui; Callaway, Edward M.; Kwon, Hyungbae; Taniguchi, Hiroki.

In: Nature neuroscience, Vol. 22, No. 3, 01.03.2019, p. 492-502.

Research output: Contribution to journalArticle

Yetman, MJ, Washburn, E, Hyun, JH, Osakada, F, Hayano, Y, Zeng, H, Callaway, EM, Kwon, H & Taniguchi, H 2019, 'Intersectional monosynaptic tracing for dissecting subtype-specific organization of GABAergic interneuron inputs', Nature neuroscience, vol. 22, no. 3, pp. 492-502. https://doi.org/10.1038/s41593-018-0322-y
Yetman, Michael J. ; Washburn, Eric ; Hyun, Jung Ho ; Osakada, Fumitaka ; Hayano, Yasufumi ; Zeng, Hongkui ; Callaway, Edward M. ; Kwon, Hyungbae ; Taniguchi, Hiroki. / Intersectional monosynaptic tracing for dissecting subtype-specific organization of GABAergic interneuron inputs. In: Nature neuroscience. 2019 ; Vol. 22, No. 3. pp. 492-502.
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