Interrogation of phosphor-specific interaction on a high-throughput label-free optical biosensor system Epic® system

Meng Wu, Shunyou Long, Anthony G. Frutos, Maryna Eichelberger, Min Li, Ye Fang

Research output: Contribution to journalArticlepeer-review

Abstract

The Epic® system, a high-throughput label-free optical biosensor system, is applied for the biochemical interrogation of phosphor-specific interactions of the 14-3-3 protein and its substrates. It has shown the capability not only for high-throughput characterization of binding rank and affinity but also for the exploration of potential interacting kinases for the substrates. A perspective of biochemical applications for diagnostics and biomarker discovery, as well as cell-based applications for endogenous receptors and viral infection characterization, are also provided.

Original languageEnglish (US)
Pages (from-to)202-210
Number of pages9
JournalJournal of Receptors and Signal Transduction
Volume29
Issue number3-4
DOIs
StatePublished - 2009

Keywords

  • 14-3-3
  • Epic.
  • Kinase
  • Label free
  • Phosphor-specific

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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