@inbook{5092d1fd52b74e65820a3a5f2448d517,
title = "Interrogating the Tumor Microenvironment by Deep Tissue Immunoprofiling",
abstract = "With the modern success of immunotherapy from chimeric antigen receptor T-cell therapy to checkpoint blockade, an understanding of the tumor microenvironment has been more essential than ever. Knowing the key immune players in the tumor can provide the basis for studying cancer host immunity and interactions governing tumor tolerance. This endeavor can be approached in a step like manner—from a broad overview with immunohistochemistry to genomic expression analysis, followed by detailed functional analysis of each cell with multiparameter flow cytometry.",
keywords = "Genomicsm, IHC, Immunoprofiling, Laser capture microdissection, Microenvironment",
author = "Nicolas Llosa",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
doi = "10.1007/978-1-0716-2014-4_10",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "139--156",
booktitle = "Methods in Molecular Biology",
}