Interpretation of the UPD/JAK/STAT morphogen gradient in Drosophila follicle cells.

Michelle Starz-Gaiano, Mariana Melani, Hans Meinhardt, Denise Montell

Research output: Contribution to journalArticle

Abstract

We are using Drosophila follicle cells to study the mechanisms that promote cell motility. Using genetics we identified a gene regulatory network that controls the dynamic pattern of activation of JAK/STAT in anterior follicle cells. Under the influence of a graded signal, Unpaired (UPD), JAK/STAT becomes activated first in a graded fashion. STAT, in turn, locally activates its own repressor, Apontic (APT), a new feedback regulator of JAK/STAT signaling. High levels of JAK/STAT also activate Slow Border Cells (SLBO), which undermines APT-mediated repression. In this way, cells that achieve a high JAK/STAT level maintain SLBO expression and form border cells, which then migrate out of the cell layer. Cells with lower JAK/STAT activity express more APT than SLBO, ultimately lose STAT activity, and remain in the follicular epithelium. To better understand how the graded signal is converted to an all-or-none decision to move or stay, we developed a mathematical model. Simulations using the model reproduce the observed dynamics of JAK/STAT expression in the wild type and in several mutant situations. By combining biological experiments and mathematical modeling, we can achieve a more sophisticated understanding of how cells interpret molecular gradients.

Original languageEnglish (US)
Pages (from-to)2917-2925
Number of pages9
JournalCell cycle (Georgetown, Tex.)
Volume8
Issue number18
StatePublished - Sep 15 2009

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Drosophila
Gene Regulatory Networks
Cell Movement
Theoretical Models
Epithelium

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Starz-Gaiano, M., Melani, M., Meinhardt, H., & Montell, D. (2009). Interpretation of the UPD/JAK/STAT morphogen gradient in Drosophila follicle cells. Cell cycle (Georgetown, Tex.), 8(18), 2917-2925.

Interpretation of the UPD/JAK/STAT morphogen gradient in Drosophila follicle cells. / Starz-Gaiano, Michelle; Melani, Mariana; Meinhardt, Hans; Montell, Denise.

In: Cell cycle (Georgetown, Tex.), Vol. 8, No. 18, 15.09.2009, p. 2917-2925.

Research output: Contribution to journalArticle

Starz-Gaiano, M, Melani, M, Meinhardt, H & Montell, D 2009, 'Interpretation of the UPD/JAK/STAT morphogen gradient in Drosophila follicle cells.', Cell cycle (Georgetown, Tex.), vol. 8, no. 18, pp. 2917-2925.
Starz-Gaiano M, Melani M, Meinhardt H, Montell D. Interpretation of the UPD/JAK/STAT morphogen gradient in Drosophila follicle cells. Cell cycle (Georgetown, Tex.). 2009 Sep 15;8(18):2917-2925.
Starz-Gaiano, Michelle ; Melani, Mariana ; Meinhardt, Hans ; Montell, Denise. / Interpretation of the UPD/JAK/STAT morphogen gradient in Drosophila follicle cells. In: Cell cycle (Georgetown, Tex.). 2009 ; Vol. 8, No. 18. pp. 2917-2925.
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