Interpretation of P53 Immunohistochemistry in Endometrial Carcinomas: Toward Increased Reproducibility

Martin Köbel, Brigitte Maria Ronnett, Naveena Singh, Robert A. Soslow, C. Blake Gilks, W. Glenn McCluggage

Research output: Contribution to journalArticle

Abstract

P53 immunohistochemistry has evolved into an accurate surrogate reflecting the underlying TP53 mutation status of a tumor, and has utility in the diagnostic workup of endometrial carcinomas. Recent work predominantly carried out in tubo-ovarian high-grade serous carcinoma has revealed 4 main patterns of p53 staining (normal/wild-Type, complete absence, overexpression, and cytoplasmic); the latter 3 patterns are variably termed abnormal/aberrant/mutation-Type and are strongly predictive of an underlying TP53 mutation. The aim of this review is to provide practical advice to pathologists regarding various aspects of p53 immunohistochemical staining. These include laboratory methods to optimize staining, a description of the different patterns of staining, advice regarding the interpretation, and reporting of p53 staining and practical uses of p53 staining in endometrial carcinoma diagnosis. Illustrations are provided to aid in the interpretational problems.

Original languageEnglish (US)
Pages (from-to)S123-S131
JournalInternational Journal of Gynecological Pathology
Volume38
Issue number1
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

Endometrial Neoplasms
Immunohistochemistry
Staining and Labeling
Mutation
Carcinoma
Neoplasms

Keywords

  • Endometrial carcinoma
  • Immunohistochemistry
  • Interpretation
  • p53
  • TP53

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Obstetrics and Gynecology

Cite this

Interpretation of P53 Immunohistochemistry in Endometrial Carcinomas : Toward Increased Reproducibility. / Köbel, Martin; Ronnett, Brigitte Maria; Singh, Naveena; Soslow, Robert A.; Gilks, C. Blake; McCluggage, W. Glenn.

In: International Journal of Gynecological Pathology, Vol. 38, No. 1, 01.01.2019, p. S123-S131.

Research output: Contribution to journalArticle

Köbel, Martin ; Ronnett, Brigitte Maria ; Singh, Naveena ; Soslow, Robert A. ; Gilks, C. Blake ; McCluggage, W. Glenn. / Interpretation of P53 Immunohistochemistry in Endometrial Carcinomas : Toward Increased Reproducibility. In: International Journal of Gynecological Pathology. 2019 ; Vol. 38, No. 1. pp. S123-S131.
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