Interplay between the temporal dynamics of the vaginal microbiota and human papillomavirus detection

Rebecca M. Brotman, Michelle D. Shardell, Pawel Gajer, J. Kathleen Tracy, Jonathan Mark Zenilman, Jacques Ravel, Patti E. Gravitt

Research output: Contribution to journalArticle

Abstract

Background. We sought to describe the temporal relationship between vaginal microbiota and human papillomavirus (HPV) detection. Methods. Thirty-two reproductive-age women self-collected midvaginal swabs twice weekly for 16 weeks (937 samples). Vaginal bacterial communities were characterized by pyrosequencing of barcoded 16S rRNA genes and clustered into 6 community state types (CSTs). Each swab was tested for 37 HPV types. The effects of CSTs on the rate of transition between HPV-negative and HPV-positive states were assessed using continuous-time Markov models. Results. Participants had an average of 29 samples, with HPV point prevalence between 58%-77%. CST was associated with changes in HPV status (P

Original languageEnglish (US)
Pages (from-to)1723-1733
Number of pages11
JournalJournal of Infectious Diseases
Volume210
Issue number11
DOIs
StatePublished - 2014

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Microbiota
rRNA Genes

Keywords

  • 16S rRNA gene analysis
  • Human papillomavirus (HPV)
  • Transition model
  • Vaginal microbiota

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Medicine(all)

Cite this

Interplay between the temporal dynamics of the vaginal microbiota and human papillomavirus detection. / Brotman, Rebecca M.; Shardell, Michelle D.; Gajer, Pawel; Tracy, J. Kathleen; Zenilman, Jonathan Mark; Ravel, Jacques; Gravitt, Patti E.

In: Journal of Infectious Diseases, Vol. 210, No. 11, 2014, p. 1723-1733.

Research output: Contribution to journalArticle

Brotman, Rebecca M. ; Shardell, Michelle D. ; Gajer, Pawel ; Tracy, J. Kathleen ; Zenilman, Jonathan Mark ; Ravel, Jacques ; Gravitt, Patti E. / Interplay between the temporal dynamics of the vaginal microbiota and human papillomavirus detection. In: Journal of Infectious Diseases. 2014 ; Vol. 210, No. 11. pp. 1723-1733.
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