@article{492a7551b6be460ca8323532bbea6c45,
title = "Intermediate-term corneal storage for penetrating keratoplasty",
abstract = "Fifty-seven penetrating keratoplasties were performed using human donor corneal material stored in modified Tissue Culture Medium 199 (TC-199). Fifty-six grafts were clear on the first postoperative day and remained clear for at least three weeks. During the follow-up period (average, 3.7 months), only four additional graft failures occurred. The reduced incidence of early graft edema is attributed to prolonged viability of corneal endothelium maintained in modified TC-199 medium prior to keratoplasty.",
author = "Stark, {Walter J.} and Maumenee, {A. Edward} and Kenyon, {Kenneth R.}",
note = "Funding Information: Despite recent advances in surgical technique and instrumentation, the failure rate for corneal transplantation ranges from 5 to 40% depending on the quality of the donor cornea and the preoperative status of the recipient eye.1About one half of graft failures occur in the immediate postoperative period,1 primarily from damage to, or inadequate preservation of, endothelial cells. Therefore, investigators have sought to improve preservation of the corneal endothelial cells during the interval between death of the donor and keratoplasty. Other than routine eye-bank storage of whole eyes at 4°C, the most acceptable method of corneal preservation is the cryopreservation process described by Capella, Kaufman, and Robbins.2 Both techniques have disadvantages, ranging from deterioration of corneal endothelial cells after prolonged whole eye storage to the exacting technique and expensive equipment required to store corneas in liquid nitrogen. Recently, McCarey and Kaufman8 reported on the use of Tissue Culture Medium 199 (TC-199) with 5% dextran and antibiotics to store rabbit corneas at 4°C. Physiologic and microscopic studies demonstrated viable endothelial cells in corneas stored up to 14 days in this tissue culture medium. We are reporting our initial clinical results using the modified TC-199 medium for intermediate-term storage of human donor corneas for penetrating keratoplasty. Additional light and electron microscopic observa-From the Wilmer Ophthalmological .Institute, Johns Hopkins Hospital, Baltimore, Maryland. This study was supported in part by grant 1-R01-EY-01302-01, the National Eye Institute (Dr. Stark). Presented in part at the Eye-Bank Association meeting, Dallas, Texas, Oct. 4, 1974. Reprint requests to Walter J. Stark, M.D., Wilmer Institute, 601 N. Broadway, Baltimore, MD 21205. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",
year = "1975",
month = may,
doi = "10.1016/0002-9394(75)90739-4",
language = "English (US)",
volume = "79",
pages = "795--802",
journal = "American journal of ophthalmology",
issn = "0002-9394",
publisher = "Elsevier USA",
number = "5",
}