Interleukin-6 prevents dexamethasone-induced myeloma cell death

James Hardin, Stewart MacLeod, Irina Grigorieva, Ruixin Chang, Bart Barlogie, Huiqing Xiao, Joshua Epstein

Research output: Contribution to journalArticle

Abstract

The effects of dexamethasone on the growth of four human multiple myeloma cell lines were studied. In addition, the effects on the expression of interleukin-6 (IL-6) and IL-6 receptor (IL-6R) genes were investigated by the use of reverse-transcriptase polymerase chain reaction. Dexamethasone (Dex) concentrations of 10-7 to 10-6 mol/L inhibited IL-6 gene expression in three of four cell lines studied, whereas the higher concentration of the hormone inhibited also IL-6R gene expression. Dex effects were modulated through the glucocorticoid receptor (GR). Dex treatment resulted in killing of sensitive cells associated with DNA fragmentation, which could be reversed by concomitant treatment with IL-6. The reversal of Dex-mediated effects by IL-6 did not result from an inhibition of GR function as measured by receptor nuclear translocation or Dex-regulated reporter gene function. These results indicate that blockage of the IL-6 signaling pathway is essential for effective myeloma cell kill by Dex.

Original languageEnglish (US)
Pages (from-to)3063-3070
Number of pages8
JournalBlood
Volume84
Issue number9
StatePublished - Nov 1 1994
Externally publishedYes

Fingerprint

Cell death
Dexamethasone
Interleukin-6
Cell Death
Interleukin-6 Receptors
Glucocorticoid Receptors
Cells
Gene expression
Genes
Gene Expression
Cell Line
DNA Fragmentation
Cytoplasmic and Nuclear Receptors
Reverse Transcriptase Polymerase Chain Reaction
Multiple Myeloma
Reporter Genes
Polymerase chain reaction
RNA-Directed DNA Polymerase
Hormones
Growth

ASJC Scopus subject areas

  • Hematology

Cite this

Hardin, J., MacLeod, S., Grigorieva, I., Chang, R., Barlogie, B., Xiao, H., & Epstein, J. (1994). Interleukin-6 prevents dexamethasone-induced myeloma cell death. Blood, 84(9), 3063-3070.

Interleukin-6 prevents dexamethasone-induced myeloma cell death. / Hardin, James; MacLeod, Stewart; Grigorieva, Irina; Chang, Ruixin; Barlogie, Bart; Xiao, Huiqing; Epstein, Joshua.

In: Blood, Vol. 84, No. 9, 01.11.1994, p. 3063-3070.

Research output: Contribution to journalArticle

Hardin, J, MacLeod, S, Grigorieva, I, Chang, R, Barlogie, B, Xiao, H & Epstein, J 1994, 'Interleukin-6 prevents dexamethasone-induced myeloma cell death', Blood, vol. 84, no. 9, pp. 3063-3070.
Hardin J, MacLeod S, Grigorieva I, Chang R, Barlogie B, Xiao H et al. Interleukin-6 prevents dexamethasone-induced myeloma cell death. Blood. 1994 Nov 1;84(9):3063-3070.
Hardin, James ; MacLeod, Stewart ; Grigorieva, Irina ; Chang, Ruixin ; Barlogie, Bart ; Xiao, Huiqing ; Epstein, Joshua. / Interleukin-6 prevents dexamethasone-induced myeloma cell death. In: Blood. 1994 ; Vol. 84, No. 9. pp. 3063-3070.
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