Interleukin-4 inhibits the expression of Kit and tryptase during stem cell factor-dependent development of human mast cells from fetal liver cells

Gunnar Nilsson, Ulla Miettinen, Teruko Ishizaka, Leonie K. Ashman, Anne Marie Irani, Lawrence B. Schwartz

Research output: Contribution to journalArticle

Abstract

Although interleukin-4 (IL-4) in mice is known to augment the proliferation of mast cells and to modulate the expression of certain mast cell protease transcripts, its effect on human mast cells is less well understood. The current study examined the effects of recombinant human IL-4 (rhuIL-4) on stem cell factor (SCF)-dependent fetal liver-derived human mast cells in liquid culture. In no case did rhuIL-4 augment proliferation of mast cells. rhuIL-4 selectively inhibited certain aspects of the development of mast cells in cultures of fetal liver cells with rhuSCF. These include lower numbers and percentages of cells expressing tryptase and surface Kit, smaller cells, and lower contents of cells for tryptase, histamine, and Kit. Development of metachromasia was not attenuated. The downregulation of Kit, the surface receptor for SCF, is probably a critical factor, because cells lacking this molecule would not be able to respond to SCF. In contrast to mast cell progenitors, mast cells already developed in vitro from fetal liver cells are relatively resistant to rhuIL-4, but are still dependent for survival on the presence of rhuSCF.

Original languageEnglish (US)
Pages (from-to)1519-1527
Number of pages9
JournalBlood
Volume84
Issue number5
StatePublished - Sep 1 1994
Externally publishedYes

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Tryptases
Stem Cell Factor
Human Development
Mast Cells
Interleukin-4
Liver
Proto-Oncogene Proteins c-kit
Histamine
Peptide Hydrolases
Cells
Molecules
Liquids
Stem Cells
Down-Regulation
Cell Culture Techniques
Cell Count
Survival

ASJC Scopus subject areas

  • Hematology

Cite this

Nilsson, G., Miettinen, U., Ishizaka, T., Ashman, L. K., Irani, A. M., & Schwartz, L. B. (1994). Interleukin-4 inhibits the expression of Kit and tryptase during stem cell factor-dependent development of human mast cells from fetal liver cells. Blood, 84(5), 1519-1527.

Interleukin-4 inhibits the expression of Kit and tryptase during stem cell factor-dependent development of human mast cells from fetal liver cells. / Nilsson, Gunnar; Miettinen, Ulla; Ishizaka, Teruko; Ashman, Leonie K.; Irani, Anne Marie; Schwartz, Lawrence B.

In: Blood, Vol. 84, No. 5, 01.09.1994, p. 1519-1527.

Research output: Contribution to journalArticle

Nilsson, G, Miettinen, U, Ishizaka, T, Ashman, LK, Irani, AM & Schwartz, LB 1994, 'Interleukin-4 inhibits the expression of Kit and tryptase during stem cell factor-dependent development of human mast cells from fetal liver cells', Blood, vol. 84, no. 5, pp. 1519-1527.
Nilsson G, Miettinen U, Ishizaka T, Ashman LK, Irani AM, Schwartz LB. Interleukin-4 inhibits the expression of Kit and tryptase during stem cell factor-dependent development of human mast cells from fetal liver cells. Blood. 1994 Sep 1;84(5):1519-1527.
Nilsson, Gunnar ; Miettinen, Ulla ; Ishizaka, Teruko ; Ashman, Leonie K. ; Irani, Anne Marie ; Schwartz, Lawrence B. / Interleukin-4 inhibits the expression of Kit and tryptase during stem cell factor-dependent development of human mast cells from fetal liver cells. In: Blood. 1994 ; Vol. 84, No. 5. pp. 1519-1527.
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