Interleukin-4 and its alternatively spliced variant (IL-4δ2) in patients with atopic asthma

Geok Teng Seah, Pei Song Gao, Julian M. Hopkin, Graham A.W. Rook

Research output: Contribution to journalArticle

Abstract

The interleukin-4 (IL-4) splice variant (IL-4δ2) is known to antagonize many biological activities of IL-4, and this challenges our understanding of the role of IL-4 in asthma. Studies that have used nonspecific antibodies, probes, and/or primers to quantify IL-4 in clinical samples would not have distinguished the expression of IL-4 from IL-4δ2. This is the first study to examine patients with chronic asthma and atopy for IL-4δ2 mRNA in their peripheral blood mononuclear cells without antigen stimulation, using a quantitative nested reverse-transcription polymerase chain reaction (RT-PCR) protocol. The median IL-4 mRNA copy number in cells from the patients with asthma was 2.8 logs higher than in a comparator group of patients with tuberculosis (p = 0.0005) and 4.5 logs higher (p = 0.0004) than in healthy control subjects. In contrast, IL-4δ2 expression in cells from patients with asthma was similar to that seen in cells from patients with tuberculosis. Hence, the median ratio of IL-4 to IL-4δ2 was 500-fold higher in the patients with asthma when compared with either patients with tuberculosis or healthy control subjects. The relative expression of IL-4 and IL-4δ2 may be a reason for the functional diversity of Th2 cells in different clinical conditions, and a hitherto unexplored mechanism for the pulmonary pathology in patients with atopic asthma.

Original languageEnglish (US)
Pages (from-to)1016-1018
Number of pages3
JournalAmerican journal of respiratory and critical care medicine
Volume164
Issue number6
DOIs
StatePublished - Sep 15 2001
Externally publishedYes

Keywords

  • Asthma
  • Atopy
  • Interleukin-4
  • Splice variant

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

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