Interleukin-3 regulates development of the 5-lipoxygenase/leukotriene C4 synthase pathway in mouse mast cells

M. Murakami, K. F. Austen, Clifton Bingham, D. S. Friend, J. F. Penrose, J. P. Arm

Research output: Contribution to journalArticle

Abstract

To study cytokine regulation of the 5-lipoxygenase (5-LO)/leukotriene (LT) synthase pathway we have developed mouse bone marrow-derived mast cells (BMMC) that minimally express each protein of the pathway by using a novel culture system, lacking interleukin (IL)-3. When mouse bone marrow cells were cultured for 5 weeks with 100 ng/ml c-kit ligand (KL) and 10 units/ml IL-10, a population of >95% mast cells was obtained. These cells generated 8.3 ± 4.5 ng of LTC4/106 cells and 8.1 ± 2.4 ng of prostaglandin (PG) D2/106 cells after IgE-dependent activation. When these BMMC were cultured for 2-5 weeks more with 100 units/ml IL-3 in the continued presence of KL and IL-10, the IgE-dependent generation of LTC4 and PGD2 increased to 212 ± 36 and 25.5 ± 8.6 ng/106 cells, respectively. The dramatic increase in the IgE- dependent generation of LTC4 in response to IL-3 was accompanied by a concomitant increase in expression of 5-LO and 5-LO-activating protein and preceded the increased expression of cytosolic phospholipase A2 and LTC4 synthase. The recognition that IL-3 up-regulates the expression of each protein of the 5-LO pathway for the generation of LTC4 contrasts with our recent finding that KL up-regulates the expression of cytosolic phospholipase A2, prostaglandin endoperoxide synthase-1, and hematopoietic PGD2 synthase and increases the IgE-dependent generation of PGD2 in BMMC developed from bone marrow with IL-3. Thus, developmentally segregated regulation of the prostanoid and cysteinyl leukotriene pathways in lineage-related committed mast cell progenitors reveals the pleiotropism of this effector cell of allergic inflammation, a cytokine/growth factor basis for preferential expression of pathways of eicosanoid biosynthesis, and the particular role of IL-3 in regulating the expression of the proteins of the 5-LO/LTC4 synthase pathway.

Original languageEnglish (US)
Pages (from-to)22653-22656
Number of pages4
JournalJournal of Biological Chemistry
Volume270
Issue number39
DOIs
StatePublished - 1995
Externally publishedYes

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Arachidonate 5-Lipoxygenase
Interleukin-3
Mast Cells
Leukotriene C4
Bone
Prostaglandin D2
Immunoglobulin E
Stem Cell Factor
Bone Marrow
Cytosolic Phospholipases A2
prostaglandin R2 D-isomerase
Interleukin-10
5-Lipoxygenase-Activating Proteins
Up-Regulation
Cytokines
Proteins
Eicosanoids
Leukotrienes
Biosynthesis
Prostaglandin-Endoperoxide Synthases

ASJC Scopus subject areas

  • Biochemistry

Cite this

Interleukin-3 regulates development of the 5-lipoxygenase/leukotriene C4 synthase pathway in mouse mast cells. / Murakami, M.; Austen, K. F.; Bingham, Clifton; Friend, D. S.; Penrose, J. F.; Arm, J. P.

In: Journal of Biological Chemistry, Vol. 270, No. 39, 1995, p. 22653-22656.

Research output: Contribution to journalArticle

Murakami, M. ; Austen, K. F. ; Bingham, Clifton ; Friend, D. S. ; Penrose, J. F. ; Arm, J. P. / Interleukin-3 regulates development of the 5-lipoxygenase/leukotriene C4 synthase pathway in mouse mast cells. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 39. pp. 22653-22656.
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abstract = "To study cytokine regulation of the 5-lipoxygenase (5-LO)/leukotriene (LT) synthase pathway we have developed mouse bone marrow-derived mast cells (BMMC) that minimally express each protein of the pathway by using a novel culture system, lacking interleukin (IL)-3. When mouse bone marrow cells were cultured for 5 weeks with 100 ng/ml c-kit ligand (KL) and 10 units/ml IL-10, a population of >95{\%} mast cells was obtained. These cells generated 8.3 ± 4.5 ng of LTC4/106 cells and 8.1 ± 2.4 ng of prostaglandin (PG) D2/106 cells after IgE-dependent activation. When these BMMC were cultured for 2-5 weeks more with 100 units/ml IL-3 in the continued presence of KL and IL-10, the IgE-dependent generation of LTC4 and PGD2 increased to 212 ± 36 and 25.5 ± 8.6 ng/106 cells, respectively. The dramatic increase in the IgE- dependent generation of LTC4 in response to IL-3 was accompanied by a concomitant increase in expression of 5-LO and 5-LO-activating protein and preceded the increased expression of cytosolic phospholipase A2 and LTC4 synthase. The recognition that IL-3 up-regulates the expression of each protein of the 5-LO pathway for the generation of LTC4 contrasts with our recent finding that KL up-regulates the expression of cytosolic phospholipase A2, prostaglandin endoperoxide synthase-1, and hematopoietic PGD2 synthase and increases the IgE-dependent generation of PGD2 in BMMC developed from bone marrow with IL-3. Thus, developmentally segregated regulation of the prostanoid and cysteinyl leukotriene pathways in lineage-related committed mast cell progenitors reveals the pleiotropism of this effector cell of allergic inflammation, a cytokine/growth factor basis for preferential expression of pathways of eicosanoid biosynthesis, and the particular role of IL-3 in regulating the expression of the proteins of the 5-LO/LTC4 synthase pathway.",
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AU - Penrose, J. F.

AU - Arm, J. P.

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AB - To study cytokine regulation of the 5-lipoxygenase (5-LO)/leukotriene (LT) synthase pathway we have developed mouse bone marrow-derived mast cells (BMMC) that minimally express each protein of the pathway by using a novel culture system, lacking interleukin (IL)-3. When mouse bone marrow cells were cultured for 5 weeks with 100 ng/ml c-kit ligand (KL) and 10 units/ml IL-10, a population of >95% mast cells was obtained. These cells generated 8.3 ± 4.5 ng of LTC4/106 cells and 8.1 ± 2.4 ng of prostaglandin (PG) D2/106 cells after IgE-dependent activation. When these BMMC were cultured for 2-5 weeks more with 100 units/ml IL-3 in the continued presence of KL and IL-10, the IgE-dependent generation of LTC4 and PGD2 increased to 212 ± 36 and 25.5 ± 8.6 ng/106 cells, respectively. The dramatic increase in the IgE- dependent generation of LTC4 in response to IL-3 was accompanied by a concomitant increase in expression of 5-LO and 5-LO-activating protein and preceded the increased expression of cytosolic phospholipase A2 and LTC4 synthase. The recognition that IL-3 up-regulates the expression of each protein of the 5-LO pathway for the generation of LTC4 contrasts with our recent finding that KL up-regulates the expression of cytosolic phospholipase A2, prostaglandin endoperoxide synthase-1, and hematopoietic PGD2 synthase and increases the IgE-dependent generation of PGD2 in BMMC developed from bone marrow with IL-3. Thus, developmentally segregated regulation of the prostanoid and cysteinyl leukotriene pathways in lineage-related committed mast cell progenitors reveals the pleiotropism of this effector cell of allergic inflammation, a cytokine/growth factor basis for preferential expression of pathways of eicosanoid biosynthesis, and the particular role of IL-3 in regulating the expression of the proteins of the 5-LO/LTC4 synthase pathway.

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