Interleukin 2 activation cytotoxic T-lymphocytes infiltrating into human metastatic melanomas

K. Itoh, A. B. Tilden, Charles M. Balch

Research output: Contribution to journalArticle

Abstract

Tumor infiltrating lymphocytes (TIL) were isolated from 22 tumors obtained from 15 patients with metastatic melanoma. In 18 of the 22 tumors, a substantial number of lymphocytes was isolated with an average lymphoid cell:tumor cell ratio of 1.26. The TIL were predominantly cytotoxic/suppressor T-lymphocytes with an average of 87% Leu4+, 61% Leu2a+, and 18% Leu3a+ cells. There were less than 2% natural killer cells, B-cells, or macrophages. An average of 3.8% (range, 51Cr release assay. rIL2-cultured TIL from all nine patients tested exhibited the highest levels of lysis against autologous tumor cells. Of the nine TIL samples, five exhibited an apparent specificity for autologous melanoma, while four specimens killed both allogenic and autologous melanoma. The ability of TIL to kill K562 targets appeared to parallel the ability to kill allogenic targets. For comparison, recombinant interleukin 2-cultured peripheral blood mononuclear cells from the same patients were assayed for cytotoxic activity against autologous and allogenic melanomas. Unlike some TIL, none of the peripheral blood mononuclear cells exhibited specificity for autologous tumor cells. In summary, TIL isolated from metastatic melanoma patients were predominantly cytotoxic T-lymphocytes with the ability to recognize and kille autologous tumor cells after in vitro culture; interleukin 2 induced proliferation of TIL and augmented their cytotoxic activity such that they eliminated autologous tumor cells.

Original languageEnglish (US)
Pages (from-to)3011-3017
Number of pages7
JournalCancer Research
Volume46
Issue number6
StatePublished - 1986
Externally publishedYes

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Tumor-Infiltrating Lymphocytes
Cytotoxic T-Lymphocytes
Interleukin-2
Melanoma
Neoplasms
Blood Cells
Lymphocyte Count
Natural Killer Cells
B-Lymphocytes
Macrophages
Lymphocytes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Interleukin 2 activation cytotoxic T-lymphocytes infiltrating into human metastatic melanomas. / Itoh, K.; Tilden, A. B.; Balch, Charles M.

In: Cancer Research, Vol. 46, No. 6, 1986, p. 3011-3017.

Research output: Contribution to journalArticle

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