Interleukin 10 suppresses phagocytic and antihyphal activities of human neutrophils

Emmanuel Roilides, Helen Katsifa, Sevasti Tsaparidou, Theodouli Stergiopoulou, Christos Panteliadis, Thomas J. Walsh

Research output: Contribution to journalArticle

Abstract

We investigated the effects of human interleukin 10 (IL-10) on the antibacterial and antifungal activities of human neutrophils (PMNs) against Staphylococcus aureus and Candida albicans. Incubation of PMNs from healthy volunteers with 20-100 ng/ml of IL-10 at 37°C for 1 h suppressed phagocytosis of serum-opsonized S. aureus (P = 0.02) and blastoconidia of C. albicans (P <0.01). In contrast, 2-100 ng/ml of IL-10 had no effect on superoxide anion production upon stimulation with phorbol myristate acetate, N-formylmethionyl leucyl phenylalanine, C. albicans blastoconidia or pseudohyphae; neither did it significantly affect conidiocidal or bactericidal activities of PMNs. However, 20-100 ng/ml of IL-10 significantly decreased PMN-induced damage of C. albicans pseudohyphae (P = 0.008). The suppression of phagocytic activity of PMNs against S. aureus and blastoconidia of C. albicans as well as the impairment of PMN-induced hyphal damage may have important implications for understanding the immunosuppressive profile of IL-10 in clinical usage.

Original languageEnglish (US)
Pages (from-to)379-387
Number of pages9
JournalCytokine
Volume12
Issue number4
DOIs
StatePublished - Apr 2000
Externally publishedYes

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Keywords

  • Candida albicans
  • Interleukin 10
  • Neutrophil
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Roilides, E., Katsifa, H., Tsaparidou, S., Stergiopoulou, T., Panteliadis, C., & Walsh, T. J. (2000). Interleukin 10 suppresses phagocytic and antihyphal activities of human neutrophils. Cytokine, 12(4), 379-387. https://doi.org/10.1006/cyto.1999.0567