Interleukin-1 is released at sites of human cutaneous allergic reactions

Bruce S. Bochner, Ernest N. Charlesworth, Lawrence M. Lichtenstein, Claudia P. Derse, Steven Gillis, Charles A. Dinarello, Robert P. Schleimer

Research output: Contribution to journalArticle

Abstract

Interleukin-1 (IL-1) promotes cell recruitment and influences allergic mediator release. We analyzed histamine, prostaglandin D2, IL-1, and leukocytes accumulating hourly for 12 hours at skin-chamber sites after local ragweed challenge in eight allergic subjects with cutaneous late-phase reactions. Ragweed induced a peak of histamine at 1 hour (p<0.02), which diminished, and then steadily increased (p<0.02). Prostaglandin D2 levels peaked by the second hour (p<0.02) and then decreased, approaching prechallenge levels by 12 hours. Leukocyte infiltration (predominantly neutrophils) was detectable 3 to 4 hours after challenge, although selective enrichment of mononuclear cells, eosinophils, and basophils was observed at later hours (p<0.02). IL-1 bioactivity was detected in fluids 10 to 12 hours after challenge but not at control sites (p<0.05). Analysis of IL-1 β levels by RIA revealed an initial peak at 1 hour of 0.90 ng/ml (p<0.02) and a second elevation of up to 0.75 ng/ml during the later hours (p<0.04). Ragweed challenge of three nonatopic subjects did not change levels of the above-mentioned mediators or cells. Bioactivity in chamber fluids from antigen-challenged sites of atopic subjects was significantly neutralized by an anti-IL-1 β antiserum, although treatment with anti-IL-1 α and anti-IL-1 β was needed for complete neutralization. IL-1 released locally during cutaneous allergic reactions may contribute to IgE-dependent cutaneous inflammation.

Original languageEnglish (US)
Pages (from-to)830-839
Number of pages10
JournalThe Journal of allergy and clinical immunology
Volume86
Issue number6 PART 1
DOIs
StatePublished - Dec 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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