Interleukin-1 induces mucus secretion from mouse intestinal explants

V. Han, J. Resau, R. Prendergast, Alan Leroy Scott, David A. Levy

Research output: Contribution to journalArticle

Abstract

Secretion of goblet cell (GC) mucus occurs during immune reactions in the gut. As human macrophages produce a substance that induces mucus secretion from lung explants, we testedthe effect of macrophage-derived factor (s) on mucus secretion from intestinal explants. Fragments of mouse duodenum were incubated with macrophage culture supernatants and purified interleukin-1 (IL-1) preparations, and the amount of mucus released was estimated by an enzyme-linked lectin assay. Both the culture supernatants and the IL-1 preparations induced dose-and time-dependent mucus release. Lipopolysaccharide-induced culture fluids were shown to contain IL-1. Thus, stimulation of mucus secretion from GC can be added to the list of biological activities attributable to IL-1.

Original languageEnglish (US)
Pages (from-to)364-365
Number of pages2
JournalInternational Archives of Allergy and Immunology
Volume82
Issue number3-4
DOIs
StatePublished - Jan 1 1987

Fingerprint

Intestinal Secretions
Mucus
Interleukin-1
Goblet Cells
Macrophages
Duodenum
Lectins
Lipopolysaccharides
Lung
Enzymes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Interleukin-1 induces mucus secretion from mouse intestinal explants. / Han, V.; Resau, J.; Prendergast, R.; Scott, Alan Leroy; Levy, David A.

In: International Archives of Allergy and Immunology, Vol. 82, No. 3-4, 01.01.1987, p. 364-365.

Research output: Contribution to journalArticle

Han, V. ; Resau, J. ; Prendergast, R. ; Scott, Alan Leroy ; Levy, David A. / Interleukin-1 induces mucus secretion from mouse intestinal explants. In: International Archives of Allergy and Immunology. 1987 ; Vol. 82, No. 3-4. pp. 364-365.
@article{aafea312c3534b47b2040853ce891f40,
title = "Interleukin-1 induces mucus secretion from mouse intestinal explants",
abstract = "Secretion of goblet cell (GC) mucus occurs during immune reactions in the gut. As human macrophages produce a substance that induces mucus secretion from lung explants, we testedthe effect of macrophage-derived factor (s) on mucus secretion from intestinal explants. Fragments of mouse duodenum were incubated with macrophage culture supernatants and purified interleukin-1 (IL-1) preparations, and the amount of mucus released was estimated by an enzyme-linked lectin assay. Both the culture supernatants and the IL-1 preparations induced dose-and time-dependent mucus release. Lipopolysaccharide-induced culture fluids were shown to contain IL-1. Thus, stimulation of mucus secretion from GC can be added to the list of biological activities attributable to IL-1.",
author = "V. Han and J. Resau and R. Prendergast and Scott, {Alan Leroy} and Levy, {David A.}",
year = "1987",
month = "1",
day = "1",
doi = "10.1159/000234228",
language = "English (US)",
volume = "82",
pages = "364--365",
journal = "International Archives of Allergy and Immunology",
issn = "1018-2438",
publisher = "S. Karger AG",
number = "3-4",

}

TY - JOUR

T1 - Interleukin-1 induces mucus secretion from mouse intestinal explants

AU - Han, V.

AU - Resau, J.

AU - Prendergast, R.

AU - Scott, Alan Leroy

AU - Levy, David A.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - Secretion of goblet cell (GC) mucus occurs during immune reactions in the gut. As human macrophages produce a substance that induces mucus secretion from lung explants, we testedthe effect of macrophage-derived factor (s) on mucus secretion from intestinal explants. Fragments of mouse duodenum were incubated with macrophage culture supernatants and purified interleukin-1 (IL-1) preparations, and the amount of mucus released was estimated by an enzyme-linked lectin assay. Both the culture supernatants and the IL-1 preparations induced dose-and time-dependent mucus release. Lipopolysaccharide-induced culture fluids were shown to contain IL-1. Thus, stimulation of mucus secretion from GC can be added to the list of biological activities attributable to IL-1.

AB - Secretion of goblet cell (GC) mucus occurs during immune reactions in the gut. As human macrophages produce a substance that induces mucus secretion from lung explants, we testedthe effect of macrophage-derived factor (s) on mucus secretion from intestinal explants. Fragments of mouse duodenum were incubated with macrophage culture supernatants and purified interleukin-1 (IL-1) preparations, and the amount of mucus released was estimated by an enzyme-linked lectin assay. Both the culture supernatants and the IL-1 preparations induced dose-and time-dependent mucus release. Lipopolysaccharide-induced culture fluids were shown to contain IL-1. Thus, stimulation of mucus secretion from GC can be added to the list of biological activities attributable to IL-1.

UR - http://www.scopus.com/inward/record.url?scp=0023275945&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023275945&partnerID=8YFLogxK

U2 - 10.1159/000234228

DO - 10.1159/000234228

M3 - Article

C2 - 3494680

AN - SCOPUS:0023275945

VL - 82

SP - 364

EP - 365

JO - International Archives of Allergy and Immunology

JF - International Archives of Allergy and Immunology

SN - 1018-2438

IS - 3-4

ER -