Interleukin-1β Converting Enzyme (ICE) like activities in apoptosis of retinal neurons after retinal ischemia-reperfusion injury

T. T. Lam, A. Gordon, Mark O M Tso

Research output: Contribution to journalArticle

Abstract

Purpose. To examine the role of interleukin-1β converting enzyme (ICE) like activities in apoptosis of inner retinal neurons. Methods. Retinal ischemia was induced in albino Lewis rats through the insertion of a cannula into the anterior chamber connected to a saline column. Intraocular pressure was kept at 110 mm Hg for 60 min. with elevated saline column. An ICE inhibitor, Ac-Tyr-Val-Ala-Asp-CMK (YVAD.CMK), at 100 μM in 0.1 M phosphate buffered saline (PBS), pH 7.4, or 0.1 M PBS for control was infused into the anterior chamber during ischemia (IVOS 35:3163-3168, 1994). Rats were killed at 18 hr after the insult to measure the apoptotic response by examining intranucleosomal fragmentation of retinal DNA with agarose gel electrophoresis or at 7 days to measure the tissue response by morphometry of the inner retinal thickness (IRT). Immunoreactivity (IM) of ICE like enzymes after the insult was followed with antibodies to CPP32 and ICH-1L. Results. YVAD.CMK inhibited the expression of the ladder pattern in DNA fragmentation study and protected the retina as measured by morphometry of IRT at 7 day. IM of CPP32 and ICH-1L. increased in the ganglion cell and inner nuclear layers at 8-18 hours after reperfusion. Conclusion. These findings are consistent with the hypothesis that ICE like enzymes may play an important role in apoptosis of inner retinal neurons.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

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Retinal Neurons
Caspase 1
Reperfusion Injury
Apoptosis
Anterior Chamber
DNA Fragmentation
Ischemia
Phosphates
Agar Gel Electrophoresis
Enzyme Inhibitors
Enzymes
Intraocular Pressure
Ganglia
Reperfusion
Retina
Antibodies
N-acetyl-tyrosyl-valyl-alanyl-aspartyl chloromethyl ketone

ASJC Scopus subject areas

  • Ophthalmology

Cite this

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title = "Interleukin-1β Converting Enzyme (ICE) like activities in apoptosis of retinal neurons after retinal ischemia-reperfusion injury",
abstract = "Purpose. To examine the role of interleukin-1β converting enzyme (ICE) like activities in apoptosis of inner retinal neurons. Methods. Retinal ischemia was induced in albino Lewis rats through the insertion of a cannula into the anterior chamber connected to a saline column. Intraocular pressure was kept at 110 mm Hg for 60 min. with elevated saline column. An ICE inhibitor, Ac-Tyr-Val-Ala-Asp-CMK (YVAD.CMK), at 100 μM in 0.1 M phosphate buffered saline (PBS), pH 7.4, or 0.1 M PBS for control was infused into the anterior chamber during ischemia (IVOS 35:3163-3168, 1994). Rats were killed at 18 hr after the insult to measure the apoptotic response by examining intranucleosomal fragmentation of retinal DNA with agarose gel electrophoresis or at 7 days to measure the tissue response by morphometry of the inner retinal thickness (IRT). Immunoreactivity (IM) of ICE like enzymes after the insult was followed with antibodies to CPP32 and ICH-1L. Results. YVAD.CMK inhibited the expression of the ladder pattern in DNA fragmentation study and protected the retina as measured by morphometry of IRT at 7 day. IM of CPP32 and ICH-1L. increased in the ganglion cell and inner nuclear layers at 8-18 hours after reperfusion. Conclusion. These findings are consistent with the hypothesis that ICE like enzymes may play an important role in apoptosis of inner retinal neurons.",
author = "Lam, {T. T.} and A. Gordon and Tso, {Mark O M}",
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N2 - Purpose. To examine the role of interleukin-1β converting enzyme (ICE) like activities in apoptosis of inner retinal neurons. Methods. Retinal ischemia was induced in albino Lewis rats through the insertion of a cannula into the anterior chamber connected to a saline column. Intraocular pressure was kept at 110 mm Hg for 60 min. with elevated saline column. An ICE inhibitor, Ac-Tyr-Val-Ala-Asp-CMK (YVAD.CMK), at 100 μM in 0.1 M phosphate buffered saline (PBS), pH 7.4, or 0.1 M PBS for control was infused into the anterior chamber during ischemia (IVOS 35:3163-3168, 1994). Rats were killed at 18 hr after the insult to measure the apoptotic response by examining intranucleosomal fragmentation of retinal DNA with agarose gel electrophoresis or at 7 days to measure the tissue response by morphometry of the inner retinal thickness (IRT). Immunoreactivity (IM) of ICE like enzymes after the insult was followed with antibodies to CPP32 and ICH-1L. Results. YVAD.CMK inhibited the expression of the ladder pattern in DNA fragmentation study and protected the retina as measured by morphometry of IRT at 7 day. IM of CPP32 and ICH-1L. increased in the ganglion cell and inner nuclear layers at 8-18 hours after reperfusion. Conclusion. These findings are consistent with the hypothesis that ICE like enzymes may play an important role in apoptosis of inner retinal neurons.

AB - Purpose. To examine the role of interleukin-1β converting enzyme (ICE) like activities in apoptosis of inner retinal neurons. Methods. Retinal ischemia was induced in albino Lewis rats through the insertion of a cannula into the anterior chamber connected to a saline column. Intraocular pressure was kept at 110 mm Hg for 60 min. with elevated saline column. An ICE inhibitor, Ac-Tyr-Val-Ala-Asp-CMK (YVAD.CMK), at 100 μM in 0.1 M phosphate buffered saline (PBS), pH 7.4, or 0.1 M PBS for control was infused into the anterior chamber during ischemia (IVOS 35:3163-3168, 1994). Rats were killed at 18 hr after the insult to measure the apoptotic response by examining intranucleosomal fragmentation of retinal DNA with agarose gel electrophoresis or at 7 days to measure the tissue response by morphometry of the inner retinal thickness (IRT). Immunoreactivity (IM) of ICE like enzymes after the insult was followed with antibodies to CPP32 and ICH-1L. Results. YVAD.CMK inhibited the expression of the ladder pattern in DNA fragmentation study and protected the retina as measured by morphometry of IRT at 7 day. IM of CPP32 and ICH-1L. increased in the ganglion cell and inner nuclear layers at 8-18 hours after reperfusion. Conclusion. These findings are consistent with the hypothesis that ICE like enzymes may play an important role in apoptosis of inner retinal neurons.

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