Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

S. F. Hurst, S. E. Kidd, C. O. Morrissey, E. Snelders, W. J G Melchers, M. V. Castelli, E. Mellado, K. Simmon, C. A. Petti, S. Richardson, Sean Xiang Zhang, A. M. Romanelli, B. L. Wickes, H. A. De Valk, C. H W Klaassen, S. Arunmozhi Balajee

Research output: Contribution to journalArticle

Abstract

Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA-3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

Original languageEnglish (US)
Pages (from-to)1562-1564
Number of pages3
JournalJournal of Clinical Microbiology
Volume47
Issue number5
DOIs
StatePublished - May 2009
Externally publishedYes

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Aspergillus fumigatus
Aspergillus
DNA Sequence Analysis
Sequence Analysis
Membrane Proteins
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Hurst, S. F., Kidd, S. E., Morrissey, C. O., Snelders, E., Melchers, W. J. G., Castelli, M. V., ... Arunmozhi Balajee, S. (2009). Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus. Journal of Clinical Microbiology, 47(5), 1562-1564. https://doi.org/10.1128/JCM.00124-09

Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus. / Hurst, S. F.; Kidd, S. E.; Morrissey, C. O.; Snelders, E.; Melchers, W. J G; Castelli, M. V.; Mellado, E.; Simmon, K.; Petti, C. A.; Richardson, S.; Zhang, Sean Xiang; Romanelli, A. M.; Wickes, B. L.; De Valk, H. A.; Klaassen, C. H W; Arunmozhi Balajee, S.

In: Journal of Clinical Microbiology, Vol. 47, No. 5, 05.2009, p. 1562-1564.

Research output: Contribution to journalArticle

Hurst, SF, Kidd, SE, Morrissey, CO, Snelders, E, Melchers, WJG, Castelli, MV, Mellado, E, Simmon, K, Petti, CA, Richardson, S, Zhang, SX, Romanelli, AM, Wickes, BL, De Valk, HA, Klaassen, CHW & Arunmozhi Balajee, S 2009, 'Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus', Journal of Clinical Microbiology, vol. 47, no. 5, pp. 1562-1564. https://doi.org/10.1128/JCM.00124-09
Hurst, S. F. ; Kidd, S. E. ; Morrissey, C. O. ; Snelders, E. ; Melchers, W. J G ; Castelli, M. V. ; Mellado, E. ; Simmon, K. ; Petti, C. A. ; Richardson, S. ; Zhang, Sean Xiang ; Romanelli, A. M. ; Wickes, B. L. ; De Valk, H. A. ; Klaassen, C. H W ; Arunmozhi Balajee, S. / Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus. In: Journal of Clinical Microbiology. 2009 ; Vol. 47, No. 5. pp. 1562-1564.
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