Interlaboratory reliability of microimmunofluorescence test for measurement of Chlamydia pneumoniae-specific immunoglobulin A and G antibody titers

Alyson J. Littman, Lisa A. Jackson, Emily White, Mark D. Thornquist, Charlotte A. Gaydos, Thomas L. Vaughan

Research output: Contribution to journalArticlepeer-review

Abstract

To evaluate the reliability of Chlamydia pneumoniae-specific immunoglobulin G (IgG) and IgA antibody titers as measured by the microimmunofluorescence (MIF) test, we compared results from 392 individuals using a standard MIF protocol at two academic laboratories. The kappas for dichotomous titers (≥16 versus <16) were 0.39 for IgA and 0.53 for IgG. Measurement error likely attenuates C. pneumoniae-disease associations; the magnitude of attenuation can be estimated from results of studies such as this one.

Original languageEnglish (US)
Pages (from-to)615-617
Number of pages3
JournalClinical and Diagnostic Laboratory Immunology
Volume11
Issue number3
DOIs
StatePublished - May 2004

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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