Abstract
Objective: To evaluate interference by the hemoglobin-based oxygen carrier Hemospan® on clinical laboratory assays. Design and methods: Interfering Hemospan concentrations were determined for general chemistry and cardiac marker analytes in pooled serum and the corresponding hemolysis index was calculated. Results: Hemospan did not interfere with 20 of 35 analytes. Hemospan produced a negative interference in serum creatinine, amylase, alkaline phosphatase, uric acid, and GGT assays and a positive interference in serum phosphate, LDH, iron, triglycerides, total protein, AST, cholesterol, magnesium, and albumin assays, and appeared to positively bias the serum cardiac troponin I (cTnI) assay only when cTnI is present in the sample. Conclusions: We present a report of assays affected by Hemospan and the threshold concentrations for interference. This study highlights the importance of interference studies in understanding the effects of hemoglobin-based oxygen carriers on results reported by the clinical laboratory.
Original language | English (US) |
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Pages (from-to) | 221-224 |
Number of pages | 4 |
Journal | Clinical Biochemistry |
Volume | 42 |
Issue number | 3 |
DOIs | |
State | Published - Feb 2009 |
Keywords
- Blood
- Chemistry
- Hemoglobin based oxygen carriers (HBOC)
- Hemospan
- Interference
- Transfusion
ASJC Scopus subject areas
- Clinical Biochemistry