Interfacial Mineral-Peptide Properties of a Mineral Binding Peptide from Osteonectin and Bone-like Apatite

Irina Matlahov, Taly Iline-Vul, Meital Abayev, Elizabeth M Y Lee, Merav Nadav-Tsubery, Keren Keinan-Adamsky, Jeffrey J Gray, Gil Goobes

Research output: Contribution to journalArticle

Abstract

Osteonectin is a regulator of bone mineralization. It interacts specifically with collagen and apatite through its N-terminal domain, inhibiting crystal growth. In this work, we investigated the interface formed between the mineral and an acidic peptide, ON29, derived from the protein's apatite binding domain. The structural properties of the peptide bound to the mineral and the mineral-peptide interface are characterized using NMR and computational methods. A biomaterial complex is formed by precipitation of the mineral in the presence of the acidic peptide. The peptide gets embedded between mineral particles, which comprise a disordered hydrated coat covering apatite-like crystals. 31P SEDRA measurements show that the peptide does not affect the overall proximity between phosphate ions in the mineral. {15N}13C REDOR measurements reveal an α-turn in the center of the free peptide, which is unchanged when it is bound to the mineral. {31P}13C REDOR and 1H-13C HETCOR measurements show that Glu/Asp carboxylates and Thr/Ala/Val side chains from ON29 are proximate to phosphate and hydroxyl groups in the mineral phases. Predictions of ON29's fold on and off hydroxyapatite crystal faces using ROSETTA-surface are used to model the molecular conformation of the peptide and its apatite-binding interface. The models constructed without bias from experimental results are consistent with NMR measurements and map out extensively the residues forming an interface with apatitic crystals. (Figure Presented).

Original languageEnglish (US)
Pages (from-to)5562-5569
Number of pages8
JournalChemistry of Materials
Volume27
Issue number16
DOIs
StatePublished - Aug 25 2015

Fingerprint

Osteonectin
Apatites
Apatite
Peptides
Minerals
Bone
Crystals
Phosphates
Nuclear magnetic resonance
Biocompatible Materials
Durapatite
Computational methods
Crystallization
Hydroxyapatite
Crystal growth
Collagen
Biomaterials
Hydroxyl Radical
Conformations
Structural properties

ASJC Scopus subject areas

  • Materials Chemistry
  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Matlahov, I., Iline-Vul, T., Abayev, M., Lee, E. M. Y., Nadav-Tsubery, M., Keinan-Adamsky, K., ... Goobes, G. (2015). Interfacial Mineral-Peptide Properties of a Mineral Binding Peptide from Osteonectin and Bone-like Apatite. Chemistry of Materials, 27(16), 5562-5569. https://doi.org/10.1021/acs.chemmater.5b01696

Interfacial Mineral-Peptide Properties of a Mineral Binding Peptide from Osteonectin and Bone-like Apatite. / Matlahov, Irina; Iline-Vul, Taly; Abayev, Meital; Lee, Elizabeth M Y; Nadav-Tsubery, Merav; Keinan-Adamsky, Keren; Gray, Jeffrey J; Goobes, Gil.

In: Chemistry of Materials, Vol. 27, No. 16, 25.08.2015, p. 5562-5569.

Research output: Contribution to journalArticle

Matlahov, I, Iline-Vul, T, Abayev, M, Lee, EMY, Nadav-Tsubery, M, Keinan-Adamsky, K, Gray, JJ & Goobes, G 2015, 'Interfacial Mineral-Peptide Properties of a Mineral Binding Peptide from Osteonectin and Bone-like Apatite', Chemistry of Materials, vol. 27, no. 16, pp. 5562-5569. https://doi.org/10.1021/acs.chemmater.5b01696
Matlahov I, Iline-Vul T, Abayev M, Lee EMY, Nadav-Tsubery M, Keinan-Adamsky K et al. Interfacial Mineral-Peptide Properties of a Mineral Binding Peptide from Osteonectin and Bone-like Apatite. Chemistry of Materials. 2015 Aug 25;27(16):5562-5569. https://doi.org/10.1021/acs.chemmater.5b01696
Matlahov, Irina ; Iline-Vul, Taly ; Abayev, Meital ; Lee, Elizabeth M Y ; Nadav-Tsubery, Merav ; Keinan-Adamsky, Keren ; Gray, Jeffrey J ; Goobes, Gil. / Interfacial Mineral-Peptide Properties of a Mineral Binding Peptide from Osteonectin and Bone-like Apatite. In: Chemistry of Materials. 2015 ; Vol. 27, No. 16. pp. 5562-5569.
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