Interactive effects of mechanical ventilation and kidney health on lung function in an in vivo mouse model

Jeffrey M. Dodd-o, Maria Hristopoulos, Daniel Scharfstein, Roy Brower, Paul Hassoun, Landon S. King, Patrice Becker, Manchang Liu, Weiwei Wang, Heitham T. Hassoun, Hamid Rabb

Research output: Contribution to journalArticle

Abstract

Dodd-o JM, Hristopoulos M, Scharfstein D, Brower R, Hassoun P, King LS, Becker P, Liu M, Wang W, Hassoun HT, Rabb H. Interactive effects of mechanical ventilation and kidney health on lung function in an in vivo mouse model. Am J Physiol Lung Cell Mol Physiol 296: L3-L11, 2009. First published October 10, 2008; doi:10.1152/ajplung.00030.2008.-We hypothesized that the influence of acute kidney injury (AKI) on the sensitivity of the lung to an injurious process varies with the severity of the injurious process. Thus, we thought that AKI would exacerbate lung injury from low degrees of lung trauma but attenuate lung injury from higher degrees of lung trauma. C57BL/6 mice underwent AKI (30-min kidney ischemia) or sham surgery, followed at 24 h by4hof spontaneous breathing (SB), mechanical ventilation with low tidal volume (7 ml/kg, LTV), or mechanical ventilation with high tidal volume (30 ml/kg, HTV). Compared with LTV, median bronchoalveolar lavage (BAL) protein leak was significantly lower with SB and greater with HTV in both sham and AKI mice. Compared with LTV, median Evans blue dye-labeled albumin extravasation in lungs (L-EBD) was also significantly lower with SB and greater with HTV. L-EBD showed a significant interaction between ventilatory mode and kidney health, such that AKI attenuated the L-EBD rise seen in HTV vs. LTV sham mice. An interaction between ventilatory mode and kidney health could also be seen in BAL neutrophil number (PMN). Thus, AKI attenuated the BAL PMN rise seen in HTV vs. LTV sham mice. These data support the presence of a complex interaction between mechanical ventilation and AKI in which the sensitivity of the lung to trauma varies with the magnitude of the trauma and may involve a modification of pulmonary neutrophil activity by AKI.

Original languageEnglish (US)
Pages (from-to)L3-L11
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume296
Issue number1
DOIs
StatePublished - Jan 1 2009

Keywords

  • Acute kidney injury
  • Evans blue dye

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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