Interactions of membrane active peptides with planar supported bilayers: An impedance spectroscopy study

Janice Lin, Jennifer Motylinski, Aram J. Krauson, William C. Wimley, Peter C. Searson, Kalina A Hristova

Research output: Contribution to journalArticle

Abstract

Membrane active peptides exert their biological effects by interacting directly with a cell's lipid bilayer membrane. These therapeutically promising peptides have demonstrated a variety of activities including antimicrobial, cytolytic, membrane translocating, and cell penetrating activities. Here, we use electrochemical impedance spectroscopy (EIS) on polymer-cushioned supported lipid bilayers constructed on single crystal silicon to study two pairs of closely related membrane active peptides selected from rationally designed, combinatorial libraries to have different activities in lipid bilayers: translocation, permeabilization, or no activity. Using EIS, we observed that binding of a membrane translocating peptide to the lipid bilayer resulted in a small decrease in membrane resistance followed by a recovery back to the original value. The recovery may be directly attributable to peptide translocation. A nontranslocating peptide did not decrease the resistance. The other pair, two membrane permeabilizing peptides, caused an exponential decrease of membrane resistance in a concentration-dependent manner. This permeabilization of the supported bilayer occurs at peptide to lipid ratios as much as 1000-fold lower than that needed to observe effects in vesicle leakage assays and gives new insights into the fundamental peptide-bilayer interactions involved in membrane permeabilization.

Original languageEnglish (US)
Pages (from-to)6088-6096
Number of pages9
JournalLangmuir
Volume28
Issue number14
DOIs
StatePublished - Apr 10 2012

Fingerprint

Peptides
peptides
Spectroscopy
impedance
membranes
Membranes
Lipid bilayers
lipids
spectroscopy
interactions
Electrochemical impedance spectroscopy
recovery
Recovery
biological effects
Silicon
Membrane Lipids
cells
Lipids
Assays
Polymers

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Interactions of membrane active peptides with planar supported bilayers : An impedance spectroscopy study. / Lin, Janice; Motylinski, Jennifer; Krauson, Aram J.; Wimley, William C.; Searson, Peter C.; Hristova, Kalina A.

In: Langmuir, Vol. 28, No. 14, 10.04.2012, p. 6088-6096.

Research output: Contribution to journalArticle

Lin, Janice ; Motylinski, Jennifer ; Krauson, Aram J. ; Wimley, William C. ; Searson, Peter C. ; Hristova, Kalina A. / Interactions of membrane active peptides with planar supported bilayers : An impedance spectroscopy study. In: Langmuir. 2012 ; Vol. 28, No. 14. pp. 6088-6096.
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