Interactions of fluorescein isothiocyanate-labeled poloxamer P188 with cultured cells

Raha Dastgheyb, Michael C. Cochran, Kenneth A. Barbee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Poloxamer 188 (P188) is a tri-block copolymer with well-established membrane sealing properties. Langmuir trough experiments using lipid monolayers have suggested that P188 interacts only with damaged membranes and that it is capable of being "squeezed out" upon the restoration of membrane integrity. Here, we investigate the interactions of P188 with cultured cells by producing a fluorescent version of P188 in which the molecule is tagged with fluorescein isothiocyanate (FITC). Uninjured cells were incubated in the presence of the fluorescent poloxamer, which was then found inside the cells. This indicates that cells are capable of internalizing the molecule such that the mechanisms by which the poloxamer is retained or released by the cell may be more complex than previously thought.

Original languageEnglish (US)
Title of host publication2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012
Pages311-312
Number of pages2
DOIs
Publication statusPublished - Jun 29 2012
Externally publishedYes
Event38th Annual Northeast Bioengineering Conference, NEBEC 2012 - Philadelphia, PA, United States
Duration: Mar 16 2012Mar 18 2012

Publication series

Name2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012

Other

Other38th Annual Northeast Bioengineering Conference, NEBEC 2012
CountryUnited States
CityPhiladelphia, PA
Period3/16/123/18/12

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ASJC Scopus subject areas

  • Bioengineering

Cite this

Dastgheyb, R., Cochran, M. C., & Barbee, K. A. (2012). Interactions of fluorescein isothiocyanate-labeled poloxamer P188 with cultured cells. In 2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012 (pp. 311-312). [6207089] (2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012). https://doi.org/10.1109/NEBC.2012.6207089