Interactions of Divalent Cations and Guanine Nucleotides at α2‐Noradrenergic Receptor Binding Sites in Bovine Brain Mechanisms

David C. U'Prichard, Solomon H. Snyder

Research output: Contribution to journalArticle

Abstract

Guanine nucleotides selectively decrease binding of the agonist ligands [3H]clonidine and [3H]epinephrine to α2‐noradrenergic receptors in calf cortex membranes. In the presence of 1.0 mM‐calcium,‐magnesium, ormanganese, inhibition by GTP of [3H]clonidine binding is reversed, so that low concentrations of GTP increase [3H]clonidine binding, whereas high GTP concentrations cause a secondary decrease. In the presence of divalent cations, low concentrations of guanyl‐5′‐yl imidodiphosphate [Gpp(NH)p], unlike GTP, do not increase binding. Differences between effects of GTP and Gpp(NH)p in the presence of divalent cations are also observed with [3H]epinephrine binding to rat and calf cortex α2‐receptors. In reversing the inhibition of α2‐agonist binding by GTP, manganese, with an ED50 of 21 μM, is much more potent and effective than magnesium or calcium. Manganese by itself increases [3H]clonidine binding by 20% an effect which is irreversible, while the interactive effects of manganese and GTP are reversible. Divalent cations also antagonize the sodium‐induced inhibition of α2‐agonist binding in calf cortex membranes, and manganese has similar ED50 values in antagonizing both sodium and GTP.

Original languageEnglish (US)
Pages (from-to)385-394
Number of pages10
JournalJournal of Neurochemistry
Volume34
Issue number2
DOIs
StatePublished - Feb 1980

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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