Interactions between Ni2+ ions and tetrapeptides containing (Asp/Lys)HisGly(l/d)His sequence

Agnieszka Matera-Witkiewicz, Justyna Brasuń, Marek Cebrat

Research output: Contribution to journalArticlepeer-review

Abstract

A series of linear tetrapeptides containing two histidyl residues in positions 2 and 4 with different chirality: DHGH, DHG(d-His), KHGH, KHG(d-His), Ac-DHGH-NH2, Ac-DHG(d-His)-NH2, Ac-KHGH-NH2, and Ac-KHG(d-His)-NH2 were synthesized, characterized and their binding properties towards Ni2+ were investigated. To establish the stoichiometry and the stability of the resulting Ni2+ complexes, potentiometric titrations were carried out. The coordination mode of the complexes formed was investigated by performing extensive spectroscopic analyses (UV-Vis, CD) in strict correlation with the potentiometric results. The effects of the nature of the first amino acid (Lys versus Asp) and of the N-terminal amino group acetylation were determined. A careful comparison of the Ni 2+ coordination abilities of the linear peptides provides a specific insight into the impact of the chirality of the C-terminal histidine residue (His4) on the metal binding properties.

Original languageEnglish (US)
Pages (from-to)3052-3058
Number of pages7
JournalPolyhedron
Volume29
Issue number16
DOIs
StatePublished - Oct 29 2010
Externally publishedYes

Keywords

  • Bioinorganic chemistry
  • Chirality
  • Histidyl residue
  • Nickel ions
  • Peptides

ASJC Scopus subject areas

  • Inorganic Chemistry
  • Materials Chemistry
  • Physical and Theoretical Chemistry

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