Interactions between entorhinal axons and target hippocampal neurons: A role for glutamate in the development of hippocampal circuitry

Mark P. Mattson, Robert E. Lee, Marvin E. Adams, Peter B. Guthrie, S. B. Kater

Research output: Contribution to journalArticle

Abstract

A coculture system consisting of input axons from entorhinal cortex explants and target hippocampal pyramidal neurons was used to demonstrate that glutamate, released spontaneously from afferent axons, can influence both dendritic geometry of target neurons and formation of presumptive synaptic sites. Dendritic outgrowth was reduced in hippocampal neurons growing on entorhinal axons when compared with neurons growing off the axons. Presumptive presynaptic sites were observed in association with hippocampal neuron dendrites and somas. HPLC analysis showed that glutamate was released from the explants in an activity-and Ca2+-dependent manner. The general glutamate receptor antagonist d-glutamylglycine significantly increased dendritic outgrowth in pyramidal neurons associated with entorhinal axons and reduced presumptive presynaptic sites. Tetrodotoxin and reduction of extracellular Ca2+ also promoted dendritic outgrowth and reduced the formation of presumptive synaptic sites. The results suggest that the neurotransmitter glutamate may play important roles in the development of hippocampal circuitry.

Original languageEnglish (US)
Pages (from-to)865-876
Number of pages12
JournalNeuron
Volume1
Issue number9
DOIs
StatePublished - 1988
Externally publishedYes

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Axons
Glutamic Acid
Neurons
Pyramidal Cells
Excitatory Amino Acid Antagonists
Entorhinal Cortex
Tetrodotoxin
Carisoprodol
Dendrites
Coculture Techniques
Neurotransmitter Agents
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Interactions between entorhinal axons and target hippocampal neurons : A role for glutamate in the development of hippocampal circuitry. / Mattson, Mark P.; Lee, Robert E.; Adams, Marvin E.; Guthrie, Peter B.; Kater, S. B.

In: Neuron, Vol. 1, No. 9, 1988, p. 865-876.

Research output: Contribution to journalArticle

Mattson, Mark P. ; Lee, Robert E. ; Adams, Marvin E. ; Guthrie, Peter B. ; Kater, S. B. / Interactions between entorhinal axons and target hippocampal neurons : A role for glutamate in the development of hippocampal circuitry. In: Neuron. 1988 ; Vol. 1, No. 9. pp. 865-876.
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