Interaction with monomeric subunit c drives insertion of ATP synthase subunit a into the membrane and primes a-c complex formation

Hannah Pierson, Eva Maria E. Uhlemann, Oleg Y. Dmitriev

Research output: Contribution to journalArticle

Abstract

Subunit a is the main part of the membrane stator of the ATP synthase molecular turbine. Subunit c is the building block of the membrane rotor. We have generated two molecular fusions of a and c subunits with different orientations of the helical hairpin of subunit c. The a/c fusion protein with correct orientation of transmembrane helices was inserted into the membrane, and co-incorporated into the F 0 complex of ATP synthase with wild type subunit c. The fused c subunit was incorporated into the c-ring tethering the ATP synthase rotor to the stator. The a/c fusion with incorrect orientation of the c-helices required wild type subunit c for insertion into the membrane. In this case, the fused c subunit remained on the periphery of the c-ring and did not interfere with rotor movement. Wild type subunit a inserted into the membrane equally well with wild type subunit c and c-ring assembly mutants that remained monomeric in the membrane. These results show that interaction with monomeric subunit c triggers insertion of subunit a into the membrane, and initiates formation of the a-c complex, the ion-translocating module of the ATP synthase. Correct assembly of the ATP synthase incorporating topologically correct fusion of subunits a and c validates using this model protein for high resolution structural studies of the ATP synthase proton channel.

Original languageEnglish (US)
Pages (from-to)38583-38591
Number of pages9
JournalJournal of Biological Chemistry
Volume286
Issue number44
DOIs
StatePublished - Nov 4 2011
Externally publishedYes

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Adenosine Triphosphate
Membranes
Fusion reactions
Rotors
Stators
Protons
Proteins
Turbines
Ions

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Interaction with monomeric subunit c drives insertion of ATP synthase subunit a into the membrane and primes a-c complex formation. / Pierson, Hannah; Uhlemann, Eva Maria E.; Dmitriev, Oleg Y.

In: Journal of Biological Chemistry, Vol. 286, No. 44, 04.11.2011, p. 38583-38591.

Research output: Contribution to journalArticle

Pierson, Hannah ; Uhlemann, Eva Maria E. ; Dmitriev, Oleg Y. / Interaction with monomeric subunit c drives insertion of ATP synthase subunit a into the membrane and primes a-c complex formation. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 44. pp. 38583-38591.
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