Interaction of yeast RNA-binding proteins Nrd1 and Nab3 with RNA polymerase II terminator elements

Kristina L. Carroll, Rodolfo Ghirlando, Jessica M. Ames, Jeffry L. Corden

Research output: Contribution to journalArticle

Abstract

Yeast RNA-binding proteins Nrd1 and Nab3 direct transcription termination of sn/snoRNA transcripts, some mRNA transcripts, and a class of intergenic and anti-sense transcripts. Recognition of Nrd1- and Nab3-binding sites is a critical first step in the termination and subsequent processing or degradation of these transcripts. In this article, we describe the purification and characterization of an Nrd1-Nab3 heterodimer. This Nrd1-Nab3 complex binds specifically to RNA sequences derived from a snoRNA terminator. The relative binding to mutant terminators correlates with the in vivo termination efficiency of these mutations, indicating that the primary specificity determinant in nonpoly(A) termination is Nrd1-Nab3 binding. In addition, several snoRNA terminators contain multiple Nrd1- and Nab3-binding sites and we show that multiple heterodimers bind cooperatively to one of these terminators in vitro. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)361-373
Number of pages13
JournalRNA
Volume13
Issue number3
DOIs
StatePublished - Mar 1 2007

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Keywords

  • RNA-binding domain
  • Transcription termination
  • snoRNA

ASJC Scopus subject areas

  • Molecular Biology

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