Abstract
Yeast RNA-binding proteins Nrd1 and Nab3 direct transcription termination of sn/snoRNA transcripts, some mRNA transcripts, and a class of intergenic and anti-sense transcripts. Recognition of Nrd1- and Nab3-binding sites is a critical first step in the termination and subsequent processing or degradation of these transcripts. In this article, we describe the purification and characterization of an Nrd1-Nab3 heterodimer. This Nrd1-Nab3 complex binds specifically to RNA sequences derived from a snoRNA terminator. The relative binding to mutant terminators correlates with the in vivo termination efficiency of these mutations, indicating that the primary specificity determinant in nonpoly(A) termination is Nrd1-Nab3 binding. In addition, several snoRNA terminators contain multiple Nrd1- and Nab3-binding sites and we show that multiple heterodimers bind cooperatively to one of these terminators in vitro. Published by Cold Spring Harbor Laboratory Press.
Original language | English (US) |
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Pages (from-to) | 361-373 |
Number of pages | 13 |
Journal | RNA |
Volume | 13 |
Issue number | 3 |
DOIs | |
State | Published - Mar 2007 |
Externally published | Yes |
Keywords
- RNA-binding domain
- Transcription termination
- snoRNA
ASJC Scopus subject areas
- Molecular Biology